User:Matthewmeisel

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	===Week 1===		===Week 1===
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		+	====Wed Jun 14====
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		+	Transformation of standard components
		+	* cultured bacteria did grow overnight
		+	* 1 mL of each tube reserved to make glycerol stock
		+	* extracted DNA from remaining 4 mL using [[Miniprep/Qiagen_kit|standard protocol]]
	====Tue Jun 13====		====Tue Jun 13====

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Revision as of 11:10, 14 June 2006

Quick link: iGEM:Harvard/2006

Contents 1 Notebook 1.1 Week 1 1.1.1 Wed Jun 14 1.1.2 Tue Jun 13 1.1.3 Mon Jun 12 2 Bibliography 3 Other projects 4 Contact info

Notebook

Week 1

Wed Jun 14

Transformation of standard components

• cultured bacteria did grow overnight
• 1 mL of each tube reserved to make glycerol stock
• extracted DNA from remaining 4 mL using standard protocol

Tue Jun 13

DNA nanostructure reaction PCR

• order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos

Transformation of standard components

Mon Jun 12

DNA nanostructure reaction PCR

• standard protocol with materials from Shawn

Transformation of standard components

Bibliography

1. Shih WM, Quispe JD, and Joyce GF. . pmid:14961116. PubMed HubMed [shih0]

• Harvard link

2. Shih WM and Spudich JA. . pmid:11278776. PubMed HubMed [shih1]

• Harvard link

3. Shih WM, Gryczynski Z, Lakowicz JR, and Spudich JA. . pmid:11007486. PubMed HubMed [shih2]

• Harvard link

Other projects

My work in Biophysics 101

Contact info

Email me: (my last name) at fas dot harvard dot edu