User:Matthewmeisel: Difference between revisions
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DNA nanostructure reaction PCR | DNA nanostructure reaction PCR | ||
* standard protocol with materials from Shawn | * [[Folding DNA nanostructures|standard protocol]] with materials from Shawn | ||
Transformation of standard components | Transformation of standard components | ||
Revision as of 09:10, 14 June 2006
Quick link: iGEM:Harvard/2006
Notebook
Week 1
Wed Jun 14
Transformation of standard components
- cultured bacteria did grow overnight
- 1 mL of each tube reserved to make glycerol stock
- extracted DNA from remaining 4 mL using standard protocol, used water for final extraction
Tue Jun 13
DNA nanostructure reaction PCR
- order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
- run on 2% agarose gel
- appears that reaction was successful (upload gel image)
Transformation of standard components
Mon Jun 12
DNA nanostructure reaction PCR
- standard protocol with materials from Shawn
Transformation of standard components
Bibliography
- Shih WM, Quispe JD, and Joyce GF. A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron. Nature. 2004 Feb 12;427(6975):618-21. DOI:10.1038/nature02307 |
- Shih WM and Spudich JA. The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. J Biol Chem. 2001 Jun 1;276(22):19491-4. DOI:10.1074/jbc.M010887200 |
- Shih WM, Gryczynski Z, Lakowicz JR, and Spudich JA. A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell. 2000 Sep 1;102(5):683-94. DOI:10.1016/s0092-8674(00)00090-8 |
Other projects
My work in Biophysics 101
Contact info
Email me: (my last name) at fas dot harvard dot edu
