User:Matthewmeisel

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Quick link: iGEM:Harvard/2006

Contents

Notebook

Week 1

Wed Jun 14

Transformation of standard components

  • cultured bacteria did grow overnight
  • 1 mL of each tube reserved to make glycerol stock
  • extracted DNA from remaining 4 mL using standard protocol, used water for final extraction

Tue Jun 13

DNA nanostructure reaction PCR

  • order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
  • run on 2% agarose gel
  • appears that reaction was successful (upload gel image)

Transformation of standard components

Mon Jun 12

DNA nanostructure reaction PCR

Transformation of standard components

Bibliography

  1. Shih WM, Quispe JD, and Joyce GF. . pmid:14961116. PubMed HubMed [shih0]
  1. Shih WM and Spudich JA. . pmid:11278776. PubMed HubMed [shih1]
  1. Shih WM, Gryczynski Z, Lakowicz JR, and Spudich JA. . pmid:11007486. PubMed HubMed [shih2]


Other projects

My work in Biophysics 101

Contact info

Email me: (my last name) at fas dot harvard dot edu

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