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| Reconstituted bovine thrombin
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| * biuret is 745 NIH units = 637 {{ug}} (1170 NIH units = 1 mg)
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| * "A suggested concentration for preparation of a stock solution is 100 units/ml. The solution should contain approximately 0.1% BSA for stability and is stable for about one week at 0-5 °C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solution in plastic tubes and store at -20 °C or below." [http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/t6634pis.pdf]
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| * Made a stock solution: reconstituted 745 NIH units in 0.490 mL of 0.1% BSA to give a working stock of 1520 units / mL = 1299 {{ug}} / mL = 20 nmol / mL = 20 {{um}} (formula weight is approximately 65 kDa [http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/t6634pis.pdf])
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| ** 0.1% BSA = 20 mg BSA in total volume of 20 mL water
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