User:Maureen McKeague: Difference between revisions

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[[Image:Photo_16.JPG|thumb|right|Maureen McKeague]]
[[Image:Photo_16.JPG|thumb|right|Maureen McKeague]]
'''Maureen McKeague'''
'''Maureen McKeague'''
<br>[http://www.stanford.edu/ Stanford University]
<br>Canada Research Chair in Genomic Chemistry, Tier II
<br>Shriram Center, Room 256
<br>Assistant Professor
<br> Mail Code 4245
<br>[https://www.mcgill.ca/pharma/facultystaff/faculty/maureen-mckeague Department of Pharmacology & Therapeutics], [https://www.mcgill.ca/medicine/ Faculty of Medicine]
<br>443 Via Ortega
<br>[https://www.mcgill.ca/chemistry/faculty/maureen-mckeague Department of Chemistry], [https://www.mcgill.ca/science/ Faculty of Science]
<br>Stanford, CA 94305
<br>[https://www.mcgill.ca/ McGill University], Canada
<br>[https://mckeague.lab.mcgill.ca/ Lab Website]
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]
I work in the [[Smolke]] lab


==Education==
==Education==
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<!--Include info about your educational background-->
* 2016-2018: Lecturer/Senior Researcher in [https://www.hest.ethz.ch/en/ Toxicology ], [https://www.ethz.ch/en.html ETH Zürich] in the [http://www.toxicology.ethz.ch/ Sturla] lab
* 2012-2016: NSERC Postdoctoral Fellow in [https://bioengineering.stanford.edu/ Bioengineering],[https://www.stanford.edu/ Stanford University] in the [http://smolkelab.weebly.com/ Smolke] lab
* 2012-2016: NSERC Postdoctoral Fellow in [https://bioengineering.stanford.edu/ Bioengineering],[https://www.stanford.edu/ Stanford University] in the [http://smolkelab.weebly.com/ Smolke] lab
* 2007-2012: PhD in [http://www5.carleton.ca/chemistry/ Chemistry],[http://carleton.ca/ Carleton University] in the [[DeRosa]] lab
* 2007-2012: PhD in [http://www5.carleton.ca/chemistry/ Chemistry],[http://carleton.ca/ Carleton University] in the [[DeRosa]] lab
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==Research interests==
==Research interests==
I am passionate about improving human health and quality of life using functional nucleic acids. Specifically, my past, current & future research aims to improve the selection and characterization of small molecule-binding aptamers and ribozymes towards the ultimate goal of developing both in vitro and in vivo biosensors for high-throughput drug screening and disease diagnosis.
 
Genomic Chemistry: I have been fascinated by nucleic acids since I learned about the basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. As such, my research bridges the chemistry of the genome to natural product production and clinical diagnostics for new applications in green chemistry and cancer therapeutics.


==Publications==
==Publications==
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#Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. [http://pubs.acs.org/doi/pdf/10.1021/acscombsci.5b00163 Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing]. ACS Comb Sci. 2016, 18 (6), 302–313.
#Maru, B., Messikommer, A., Huang, M., Seipel, K., Kovecses, O., Valk, P.J.M., Theocharides, A.P.A., Mercier, F.E., Pabst, T., McKeague, M., Luedtke, N. [https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791(23)00358-0?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2666379123003580%3Fshowall%3Dtrue PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy.] <i>Cell Reports Medicine.</i> 2023, 4, 101191362.
#McKeague, M.; Wong, R.S.; Smolke, C.D. [http://nar.oxfordjournals.org/content/early/2016/03/10/nar.gkw151.abstract?keytype=ref&ijkey=P6DDJUKcmpn8XIM Opportunities in the design and application of RNA for gene expression control] NAR, 2016, 44 (7),2987-2999
#Jiang, Y.; Mingard, C.; Huber, S.; Takhaveev, V.; McKeague, M.; Kizaki, S.; Ziegler, N.; Schneider, M.; Hürlimann, V.; Hoeng, J.; Sierro, N.; Ivanov, N.; Sturla, S.J. [https://pubs.acs.org/doi/10.1021/acscentsci.2c01100 Quantification and Mapping of Alkylation in the Human Genome Reveal Single Nucleotide Resolution Precursors of Mutational Signatures.] <i>ACS Central Science.</i> 2023, 3, 362–372.
#McKeague, M.; Wang, Y-H.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00518 In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control] ACS Chem. Biol, 2015, 10 (11), 2463–2467.
#McKeague, M.; Calzada, V.; Cerchia, L.; DeRosa, M.C.; Heemstra, J.M.; Janjic, N.; Johnson, P.E.; Kraus, L.; Limson, J.; Mayer, G.; Nilsen-Hamilton, M.; Porciani, D.; Sharma, T.K.; Suess, B.; Tanner, J.A.; Shigdar, S. [http://japtamers.co.uk/the-minimum-aptamer-publication-standards-maps-guidelines-for-de-novo-aptamer-selection/ The minimum aptamer publication standards (MAPS guidelines) for de novo aptamer selection.] <i>Aptamers.</i> 2022.
#McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. [http://link.springer.com/article/10.1007%2Fs00239-015-9708-6 Analysis of In Vitro Aptamer Selection Parameters]. J Mol Evol. 2015,  81(5), 150-161.
#Forder, M.; McKeague, M. [https://msurjonline.mcgill.ca/article/view/176 Tuning Aptamer-Switching for Biosensing Malarial Proteins.] <i> MSURJ.</i> 2022.
#Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an01075f#!divAbstract An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers], Analyst 2015, 140 (8) 6643–6651.
#Robinson, K.A.; Lee, S.Y.; Friedman, S.; Christiaans, E.; McKeague, M.; Pavelka, L.; Sirjoosingh, P.  [https://www.sciencedirect.com/science/article/pii/S0361476X22000145 You know what, I can do this”: Heterogeneous Joint Trajectories of Expectancy for Success and Attainment Value in Chemistry.] <i>Contemporary Educational Psychology. </i> 2022. 69, 102055.
#McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.[http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b02102 Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation], Analytical Chemistry, 2015, 87 (17), 8608–8612.
#Maru, B.; Nadeau, L.; McKeague, M. [https://pubs.acs.org/doi/10.1021/acsptsci.1c00188 Enhancing CAR-T Cell Therapy with Functional Nucleic Acids.] <i>ACS Pharmacol. Transl. Sci.</i> 2021,4, 6, 1716–17273.
#Bechu, A.; Liao, J.; Huang, C.; Ahn, C.; McKeague, M.; Ghoshal, S.; Moores, A. [https://pubs.acs.org/doi/10.1021/acsanm.1c01659 Cadmium-Containing Quantum Dots Used in Electronic Displays: Implications for Toxicity and Environmental Transformations.] <i>ACS Appl. Nano Mater.</i> 2021. 8: 8417-8428.
#Hirka, S; McKeague, M. [http://japtamers.co.uk/quantification-of-aptamer-protein-binding-with-fluorescence-anisotropy/ Quantification of aptamer-protein binding with fluorescence anisotropy] <i>Aptamers</i>. 2021, 5.
#Krebs, J.; McKeague, M. [https://pubs.acs.org/doi/10.1021/acs.chemrestox.0c00260 Green toxicology: connecting green chemistry and modern toxicology] <i>Chemical Research in Toxicology</i>. 2020, 33, 2919–2931.
#Mingard, C.; Wu, J.; McKeague, M.; Sturla, S.J. [https://pubs.rsc.org/en/content/articlelanding/2020/cs/d0cs00647e#!divAbstract Next-generation DNA damage sequencing] <i>Chemical Society Reviews</i>. 2020, 49, 7354-7377.
#Wang, L.; McKeague, M. [http://japtamers.co.uk/aptamers-in-the-pursuit-of-covid-19-management/ Aptamers in the pursuit of COVID-19 management] <i>Aptamers</i>. 2020, 4, 1-2.
#Oteng, E.K.; Gu, W.; McKeague, M. [https://www.nature.com/articles/s41598-020-66537-1 High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes] <i>Scientific Reports</i>. 2020, 10, 9706.
#Xiang, J.S.; Kaplan, M.; Dykstra, P.; Hinks, M.; McKeague, M.; Smolke, C.D. [https://www.nature.com/articles/s41467-019-12334-y Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq and FACS-Seq] <i>Nature Communications</i>. 2019. 10, 4327.
#Shigdar, S.; Johnson, P.E.; Pietruschka, G.; Legen, T.; Mayer, G.; McKeague, M. [http://japtamers.co.uk/wp-content/uploads/2019/07/Shigdar.pdf Aptamers 2019-a conference update] <i>Aptamers</i>. 2019, 3, 1-3.
#Smith, M.; McKeague, M.; DeRosa, M.C. [https://www.sciencedirect.com/science/article/pii/S2215016119300172?via%3Dihub Synthesis, Transfer, and Characterization of Core-Shell Gold-Coated Magnetic Nanoparticles] <i>MethodsX</i>. 2019.
#Kraus, A.; McKeague, M.; Seiwert, N.; Nagel, G.; Minneker, V.; Geisen, S. Zeigler, N.; Trantakis, I.A.; Kaina, B.; Thomas, A.D.; Sturla, S.J.; Fahrer, J. [https://link.springer.com/article/10.1007%2Fs00204-018-2355-0 Immunological and mass spectrometry-based approaches to determine thresholds of the mutagenic DNA adduct O6-methylguanine in vivo], <i>Arch Toxicol</i>. 2019, 93, 559–572.
#Lam, J.; McKeague, M. [https://www.sciencedirect.com/science/article/pii/S0955286317309890 Dietary modulation of mitochondrial DNA damage: Implications in ageing and associated diseases] <i>The Journal of Nutritional Biochemistry</i>. 2019, 63, 1–10.
#McKeague, M.; Otto, C.; Angelov, T.; Räz, M.H.; Sturla, S.J. 2018. [https://pubs.acs.org/doi/abs/10.1021/acschembio.8b00446 Base-pairing partner modulates alkylguanine alkyltransferase] <i>ACS Chemical Biology</i>. 2018, 13 (9), 2534-2541.
#Wu, J.; McKeague, M.; Sturla, S.J. [https://pubs.acs.org/doi/10.1021/jacs.8b03715 Nucleotide-resolution genome-wide mapping of oxidative DNA damage by click-code-seq], <i>JACS</i>. 2018, 140 (31), 9783-9787.
#DeGirolamo, A.;, McKeague, M.; Pascale, M.; Cortese, M.; DeRosa, M.C. [https://books.google.ca/books?hl=en&lr=&id=DrFyDwAAQBAJ&oi=fnd&pg=PA85&dq=info:a7TuI7LqOY8J:scholar.google.com&ots=MjRE5u67jY&sig=gXsvcNDBnWsvKRON74WnhAKW4qk&redir_esc=y#v=onepage&q&f=false Chapter 3: Immobilization of aptamers on substrates]. Aptamers for Analytical Applications: Affinity Optimization and Method Design. Wiley-VCH Verlag GmbH & Co. (Ed) 2018, 85-126.
#Tanner, J.A.; Ismail, S.I.; Shigdar, S.; DeRosa, M.C.; Hahn, U.; Johnson, P.E.; Suess, B.; McKeague, M. [http://japtamers.co.uk/aptamers-2018-a-conference-update/ Aptamers 2018-a conference update] <i>Aptamers</i>. 2018, 2, 52-54.
#Dahlmann, H.A.; Berger, F.D.; Kung, R.W.; Wyss, L.A.; Gubler, I.; McKeague, M.; Wetmore, S.D.; Sturla, S.J. [https://onlinelibrary.wiley.com/doi/pdf/10.1002/hlca.201800066 Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts] <i>Helvetica Chimica Acta</i>.
#Dörsam, B.; Seiwert, N.; Foersch, S.; Stroh, S.; Nagel, G.; Begaliew, G.; Diehl, E.; Kraus, A.; McKeague, M.; Minneker, V.; Roukos, V.; Reißig, V.; Waisman, A.; Moehler, M.; Stier, A.; Mangerich, A.; Dantzer, F.; Kaina, B.; Fahrer, J. [http://www.pnas.org/content/early/2018/04/03/1712345115 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression], <i>PNAS</i>. 2018, 115, E4061-E4070.
#Awwad, A.M.; McKeague, M. [http://japtamers.co.uk/wp-content/uploads/2018/02/Awwad.pdf Riboswitches and synthetic aptamers: a head-to-head comparison] <i>Aptamers</i>. 2018, 2, 1-10.
#Henri, J.; McKeague, M.; Johnson, P.E.; Suess, B.; Nakamura, Y.; Nilsen-Hamilton, M.; Pastor, F.; Hahn, U.; Bunka, D.; Shigdar, S. [http://japtamers.co.uk/wp-content/uploads/2018/02/Henri.pdf Aptamers 2017 at Oxford] <i>Aptamers</i>. 2018, 2, 11-14.
#Ruscito, A.; McConnell, E.M.; Koudrina, A.; Ranganathan, V.; Mattice, C.; Hunt, V.; McKeague, M.; DeRosa, M.C. [http://onlinelibrary.wiley.com/doi/10.1002/cpch.28/abstract In vitro selection and characterization of DNA aptamers to a small molecule target] <i>Current Protocols in Chemical Biology</i>. 2017, 9, 233-268.
#McKeague, M. [http://www.mdpi.com/1422-0067/18/10/2212/htm Aptamers for DNA damage and repair] <i>International Journal of Molecular Sciences</i>. 2017, 18 (10), 2212.
#Alsaafin, A.; McKeague, M. [http://www.sciencedirect.com/science/article/pii/S0956566317301173 Functional nucleic acids as in vivo metabolite and ion biosensors] <i>Biosensors and Bioelectronics</i>. 2017, 94, 94–106.
#McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/8/11/336/htm Comparison of in-solution biorecognition properties of aptamers against ochratoxin A], <i>Toxins</i>. 2016, 8 (11), 336-348.
#Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. [http://www.cell.com/cell-systems/fulltext/S2405-4712(16)30327-1 Design and construction of generalizable RNA-protein hybrid controllers by level-matched genetic signal amplification] <i>Cell Systems</i>. 2016, 3 (6), 549–562.
#McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/S1096717616300738 Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines] <i>Metabolic Engineering</i>. 2016, 38, 191–203.
#Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. [http://pubs.acs.org/doi/pdf/10.1021/acscombsci.5b00163 Screening and Identification of DNA aptamers to tyramine using in vitro selection and high-throughput sequencing]. <i>ACS Combinatorial Science</i>. 2016, 18 (6), 302–313.
#McKeague, M.; Wong, R.S.; Smolke, C.D. [http://nar.oxfordjournals.org/content/early/2016/03/10/nar.gkw151.abstract?keytype=ref&ijkey=P6DDJUKcmpn8XIM Opportunities in the design and application of RNA for gene expression control] <i>Nucleic Acids Research</i>. 2016, 44 (7),2987-2999
#McKeague, M.; Wang, Y-H.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00518 In vitro screening and in silico modeling of RNA-based gene expression control] <i>ACS Chemical Biology</i>. 2015, 10 (11), 2463–2467.
#McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. [http://link.springer.com/article/10.1007%2Fs00239-015-9708-6 Analysis of in vitro aptamer selection parameters]. <i>Journal of Molecular Evolution</i>. 2015,  81(5), 150-161.
#Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an01075f#!divAbstract An in solution assay for parallel interrogation of structure and affinity of small molecule-binding aptamers], <i>Analyst</i>. 2015, 140 (8) 6643–6651.
#McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.[http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b02102 Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation], <i>Analytical Chemistry</i>. 2015, 87 (17), 8608–8612.
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.  
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.  
#McKeague, M.; DeRosa, M.C. [http://smithandfranklin.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology], Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.  
#McKeague, M.; DeRosa, M.C. [http://www.researcherslinks.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the development of transformative molecular recognition technology], <i>Aptamers and Synthetic Antibodies</i>. 2014, 1 (1), 12-16.  
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and characterization of a novel DNA aptamer For label-free fluorescence biosensing of ochratoxin A], <i>Toxins</i>. 2014, 6 (8), 2435-2452.
#Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/ac5001527 Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform], Analytical Chemistry, 2014, 86 (7), 3273–3278.
#Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/ac5001527 Kinetic and equilibrium binding characterization of aptamers to small molecules using a label-free, sensitive, and scalable Platform], <i>Analytical Chemistry</i>. 2014, 86 (7), 3273–3278.
#Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. [http://www.tandfonline.com/doi/abs/10.1080/07391102.2013.786381?queryID=%24%7BresultBean.queryID%7D#.U72ysvldWv8 Development and Application of Crop Exudate Specific Aptamers], Journal of Biomolecular Structure and Dynamics, 2013, 31 (1), 89.
#Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. [http://www.tandfonline.com/doi/abs/10.1080/07391102.2013.786381?queryID=%24%7BresultBean.queryID%7D#.U72ysvldWv8 Development and application of crop exudate specific aptamers], <i>Journal of Biomolecular Structure and Dynamics</i>. 2013, 31 (1), 89.
#McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra43893g#!divAbstract/ Development of a DNA aptamer for direct and selective homocysteine detection in human serum], RSC Advances, 2013, 3 (46), 24415-24422.
#McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra43893g#!divAbstract/ Development of a DNA aptamer for direct and selective homocysteine detection in human serum], <i>RSC Advances</i>. 2013, 3 (46), 24415-24422.
#McKeague, M.; DeRosa, M.C. [http://www.hindawi.com/journals/jna/2012/748913/cta/ Challenges and Opportunities for Small Molecule Aptamer Development], Journal of Nucleic Acids, 2012.  
#McKeague, M.; DeRosa, M.C. [http://www.hindawi.com/journals/jna/2012/748913/cta/ Challenges and opportunities for small molecule aptamer development], <i>Journal of Nucleic Acids</i>. 2012.  
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. Database: Journal of Biological Databases and Curation. 2012.   
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. <i>Database: Journal of Biological Databases and Curation</i>. 2012.   
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in Aptamer-Based Biosensors for Food Safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in aptamer-based biosensors for food safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column]. Food Chem. 2011, 127 (3), 1378-1384.
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column]. <i>Food Chemistry</i>. 2011, 127 (3), 1378-1384.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers], Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and initial binding assessment of fumonisin B(1) aptamers], <i>International Journal of Molecular Sciences</i>. 2010, 11 (12), 4864-4881.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers], RNA. 2010, 16 (11), 2252-2262.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational approaches toward the design of pools for the in vitro selection of complex aptamers], <i>RNA</i>. 2010, 16 (11), 2252-2262.


==Useful links==
==Useful links==
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance]
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance] and [https://cen.acs.org/articles/88/i44/Dancing-Chemists-Old-Butter-Asparagus.html C&EN]
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*[[Media:CVMCKEAGUE.pdf|Full curriculum vitae]]
*[http://www.oligotherapeutics.org/interview-with-maureen-mckeague-phd/ Interview with the Oligonucleotide Therapeutics Society]
*[http://www.oligotherapeutics.org/interview-with-maureen-mckeague-phd/ Interview with the Oligonucleotide Therapeutics Society]
*[http://aptamersociety.org/about-insoap/ International Society on Aptamers]
*[http://aptamersociety.org/about-insoap/ International Society on Aptamers]
*Building a search engine for aptamers! [http://www.freebase.com/base/aptamer Aptamer Base]
*[http://nserc-promote.research.mcgill.ca/ Training Program in Nucleic Acids (NSERC)]
*2015 Analytical Chemistry [http://pubs.acs.org/iapps/liveslides/pages/index.htm?mscNo=acs.analchem.5b02102 LiveSlides ]
*[https://www.mcgill.ca/chemistry/department/outreach Chemistry Outreach]
*[https://www.mcgill.ca/pharma/newsevents/win4science Women in Science (Win4Science), McGill]

Latest revision as of 16:21, 17 September 2023

Contact Info

Maureen McKeague

Maureen McKeague
Canada Research Chair in Genomic Chemistry, Tier II
Assistant Professor
Department of Pharmacology & Therapeutics, Faculty of Medicine
Department of Chemistry, Faculty of Science
McGill University, Canada
Lab Website
Email me through OpenWetWare

Education

Research interests

Genomic Chemistry: I have been fascinated by nucleic acids since I learned about the basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. As such, my research bridges the chemistry of the genome to natural product production and clinical diagnostics for new applications in green chemistry and cancer therapeutics.

Publications

  1. Maru, B., Messikommer, A., Huang, M., Seipel, K., Kovecses, O., Valk, P.J.M., Theocharides, A.P.A., Mercier, F.E., Pabst, T., McKeague, M., Luedtke, N. PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy. Cell Reports Medicine. 2023, 4, 101191362.
  2. Jiang, Y.; Mingard, C.; Huber, S.; Takhaveev, V.; McKeague, M.; Kizaki, S.; Ziegler, N.; Schneider, M.; Hürlimann, V.; Hoeng, J.; Sierro, N.; Ivanov, N.; Sturla, S.J. Quantification and Mapping of Alkylation in the Human Genome Reveal Single Nucleotide Resolution Precursors of Mutational Signatures. ACS Central Science. 2023, 3, 362–372.
  3. McKeague, M.; Calzada, V.; Cerchia, L.; DeRosa, M.C.; Heemstra, J.M.; Janjic, N.; Johnson, P.E.; Kraus, L.; Limson, J.; Mayer, G.; Nilsen-Hamilton, M.; Porciani, D.; Sharma, T.K.; Suess, B.; Tanner, J.A.; Shigdar, S. The minimum aptamer publication standards (MAPS guidelines) for de novo aptamer selection. Aptamers. 2022.
  4. Forder, M.; McKeague, M. Tuning Aptamer-Switching for Biosensing Malarial Proteins. MSURJ. 2022.
  5. Robinson, K.A.; Lee, S.Y.; Friedman, S.; Christiaans, E.; McKeague, M.; Pavelka, L.; Sirjoosingh, P. You know what, I can do this”: Heterogeneous Joint Trajectories of Expectancy for Success and Attainment Value in Chemistry. Contemporary Educational Psychology. 2022. 69, 102055.
  6. Maru, B.; Nadeau, L.; McKeague, M. Enhancing CAR-T Cell Therapy with Functional Nucleic Acids. ACS Pharmacol. Transl. Sci. 2021,4, 6, 1716–17273.
  7. Bechu, A.; Liao, J.; Huang, C.; Ahn, C.; McKeague, M.; Ghoshal, S.; Moores, A. Cadmium-Containing Quantum Dots Used in Electronic Displays: Implications for Toxicity and Environmental Transformations. ACS Appl. Nano Mater. 2021. 8: 8417-8428.
  8. Hirka, S; McKeague, M. Quantification of aptamer-protein binding with fluorescence anisotropy Aptamers. 2021, 5.
  9. Krebs, J.; McKeague, M. Green toxicology: connecting green chemistry and modern toxicology Chemical Research in Toxicology. 2020, 33, 2919–2931.
  10. Mingard, C.; Wu, J.; McKeague, M.; Sturla, S.J. Next-generation DNA damage sequencing Chemical Society Reviews. 2020, 49, 7354-7377.
  11. Wang, L.; McKeague, M. Aptamers in the pursuit of COVID-19 management Aptamers. 2020, 4, 1-2.
  12. Oteng, E.K.; Gu, W.; McKeague, M. High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes Scientific Reports. 2020, 10, 9706.
  13. Xiang, J.S.; Kaplan, M.; Dykstra, P.; Hinks, M.; McKeague, M.; Smolke, C.D. Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq and FACS-Seq Nature Communications. 2019. 10, 4327.
  14. Shigdar, S.; Johnson, P.E.; Pietruschka, G.; Legen, T.; Mayer, G.; McKeague, M. Aptamers 2019-a conference update Aptamers. 2019, 3, 1-3.
  15. Smith, M.; McKeague, M.; DeRosa, M.C. Synthesis, Transfer, and Characterization of Core-Shell Gold-Coated Magnetic Nanoparticles MethodsX. 2019.
  16. Kraus, A.; McKeague, M.; Seiwert, N.; Nagel, G.; Minneker, V.; Geisen, S. Zeigler, N.; Trantakis, I.A.; Kaina, B.; Thomas, A.D.; Sturla, S.J.; Fahrer, J. Immunological and mass spectrometry-based approaches to determine thresholds of the mutagenic DNA adduct O6-methylguanine in vivo, Arch Toxicol. 2019, 93, 559–572.
  17. Lam, J.; McKeague, M. Dietary modulation of mitochondrial DNA damage: Implications in ageing and associated diseases The Journal of Nutritional Biochemistry. 2019, 63, 1–10.
  18. McKeague, M.; Otto, C.; Angelov, T.; Räz, M.H.; Sturla, S.J. 2018. Base-pairing partner modulates alkylguanine alkyltransferase ACS Chemical Biology. 2018, 13 (9), 2534-2541.
  19. Wu, J.; McKeague, M.; Sturla, S.J. Nucleotide-resolution genome-wide mapping of oxidative DNA damage by click-code-seq, JACS. 2018, 140 (31), 9783-9787.
  20. DeGirolamo, A.;, McKeague, M.; Pascale, M.; Cortese, M.; DeRosa, M.C. Chapter 3: Immobilization of aptamers on substrates. Aptamers for Analytical Applications: Affinity Optimization and Method Design. Wiley-VCH Verlag GmbH & Co. (Ed) 2018, 85-126.
  21. Tanner, J.A.; Ismail, S.I.; Shigdar, S.; DeRosa, M.C.; Hahn, U.; Johnson, P.E.; Suess, B.; McKeague, M. Aptamers 2018-a conference update Aptamers. 2018, 2, 52-54.
  22. Dahlmann, H.A.; Berger, F.D.; Kung, R.W.; Wyss, L.A.; Gubler, I.; McKeague, M.; Wetmore, S.D.; Sturla, S.J. Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts Helvetica Chimica Acta.
  23. Dörsam, B.; Seiwert, N.; Foersch, S.; Stroh, S.; Nagel, G.; Begaliew, G.; Diehl, E.; Kraus, A.; McKeague, M.; Minneker, V.; Roukos, V.; Reißig, V.; Waisman, A.; Moehler, M.; Stier, A.; Mangerich, A.; Dantzer, F.; Kaina, B.; Fahrer, J. PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression, PNAS. 2018, 115, E4061-E4070.
  24. Awwad, A.M.; McKeague, M. Riboswitches and synthetic aptamers: a head-to-head comparison Aptamers. 2018, 2, 1-10.
  25. Henri, J.; McKeague, M.; Johnson, P.E.; Suess, B.; Nakamura, Y.; Nilsen-Hamilton, M.; Pastor, F.; Hahn, U.; Bunka, D.; Shigdar, S. Aptamers 2017 at Oxford Aptamers. 2018, 2, 11-14.
  26. Ruscito, A.; McConnell, E.M.; Koudrina, A.; Ranganathan, V.; Mattice, C.; Hunt, V.; McKeague, M.; DeRosa, M.C. In vitro selection and characterization of DNA aptamers to a small molecule target Current Protocols in Chemical Biology. 2017, 9, 233-268.
  27. McKeague, M. Aptamers for DNA damage and repair International Journal of Molecular Sciences. 2017, 18 (10), 2212.
  28. Alsaafin, A.; McKeague, M. Functional nucleic acids as in vivo metabolite and ion biosensors Biosensors and Bioelectronics. 2017, 94, 94–106.
  29. McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. Comparison of in-solution biorecognition properties of aptamers against ochratoxin A, Toxins. 2016, 8 (11), 336-348.
  30. Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. Design and construction of generalizable RNA-protein hybrid controllers by level-matched genetic signal amplification Cell Systems. 2016, 3 (6), 549–562.
  31. McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines Metabolic Engineering. 2016, 38, 191–203.
  32. Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. Screening and Identification of DNA aptamers to tyramine using in vitro selection and high-throughput sequencing. ACS Combinatorial Science. 2016, 18 (6), 302–313.
  33. McKeague, M.; Wong, R.S.; Smolke, C.D. Opportunities in the design and application of RNA for gene expression control Nucleic Acids Research. 2016, 44 (7),2987-2999
  34. McKeague, M.; Wang, Y-H.; Smolke, C.D. In vitro screening and in silico modeling of RNA-based gene expression control ACS Chemical Biology. 2015, 10 (11), 2463–2467.
  35. McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. Analysis of in vitro aptamer selection parameters. Journal of Molecular Evolution. 2015, 81(5), 150-161.
  36. Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. An in solution assay for parallel interrogation of structure and affinity of small molecule-binding aptamers, Analyst. 2015, 140 (8) 6643–6651.
  37. McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation, Analytical Chemistry. 2015, 87 (17), 8608–8612.
  38. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  39. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the development of transformative molecular recognition technology, Aptamers and Synthetic Antibodies. 2014, 1 (1), 12-16.
  40. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and characterization of a novel DNA aptamer For label-free fluorescence biosensing of ochratoxin A, Toxins. 2014, 6 (8), 2435-2452.
  41. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and equilibrium binding characterization of aptamers to small molecules using a label-free, sensitive, and scalable Platform, Analytical Chemistry. 2014, 86 (7), 3273–3278.
  42. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and application of crop exudate specific aptamers, Journal of Biomolecular Structure and Dynamics. 2013, 31 (1), 89.
  43. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances. 2013, 3 (46), 24415-24422.
  44. McKeague, M.; DeRosa, M.C. Challenges and opportunities for small molecule aptamer development, Journal of Nucleic Acids. 2012.
  45. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  46. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in aptamer-based biosensors for food safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  47. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column. Food Chemistry. 2011, 127 (3), 1378-1384.
  48. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and initial binding assessment of fumonisin B(1) aptamers, International Journal of Molecular Sciences. 2010, 11 (12), 4864-4881.
  49. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational approaches toward the design of pools for the in vitro selection of complex aptamers, RNA. 2010, 16 (11), 2252-2262.

Useful links

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