User:Maureen McKeague: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(47 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Contact Info==
==Contact Info==
[[Image:OWWEmblem.png|thumb|right|Maureen McKeague (an artistic interpretation)]]
[[Image:Photo_16.JPG|thumb|right|Maureen McKeague]]
<br>'''Maureen McKeague'''
'''Maureen McKeague'''
<br>[http://carleton.ca/ Carleton University]
<br>[http://www.stanford.edu/ Stanford University]
<br>1125 Colonel By Drive
<br>Shriram Center, Room 256
<br>Ottawa, ON, Canada
<br> Mail Code 4245
<br>443 Via Ortega
<br>Stanford, CA 94305
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]


I work in the [[DeRosa]] lab at Carleton University. 
I work in the [[Smolke]] lab


==Education==
==Education==
<!--Include info about your educational background-->
<!--Include info about your educational background-->
* 2007-Current: PhD in [http://www5.carleton.ca/chemistry/ Chemistry] at [http://carleton.ca/ Carleton University]
* 2007-2012: PhD in [http://www5.carleton.ca/chemistry/ Chemistry],[http://carleton.ca/ Carleton University] in the [[DeRosa]] lab
* 2003-2007: BSc in [http://www1.carleton.ca/biochem/research/biotechnology/ Biochemistry and Biotechnology] from [http://carleton.ca/ Carleton University]
* 2003-2007: BSc in [http://www1.carleton.ca/biochem/research/biotechnology/ Biochemistry and Biotechnology], [http://carleton.ca/ Carleton University]


==Research interests==
==Research interests==
<!-- Feel free to add brief descriptions to your research interests as well -->
My past, current, and future research aims to improve the workflow of the selection and characterization of small molecule-binding aptamers towards the ultimate goal of developing both in vitro and in vivo biosensors that link important metabolite concentrations to readily detectable signals for high throughput screening and diagnostics.
My PhD research involves improving the Systematic Evolution of Ligands by Exponential enrichment (SELEX) process using a combination of experimental and computational methods. I am also applying the improved SELEX technique to produce aptamers that can recognize and bind to mycotoxins (fungal metabolites) with high affinity. It is estimated that at least 25% of the grain produced worldwide is contaminated with mycotoxins. In Canada, three mycotoxins of major concern include fumonisin B1 (FB1), deoxynivalenol (DON) and ochratoxin A (OA). Mycotoxin exposure leads to a variety of pathologies including vomiting (DON);  kidney disease (OA, FB1); neurological disorders (FB1); disease of the lung and liver (FB1); cancer (OA, FB1) and death (FB1, OA). While traditional food safety testing techniques to detect mycotoxins exist; there is a need for more rapid and cost-effective approaches. We expect that the relatively new technology of aptamers is a viable alternative for use in food testing, specifically for mycotoxin detection.


==Publications==
==Publications==
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
#McKeague, M.; DeRosa, M.C. [http://smithandfranklin.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology], Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
#Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/ac5001527 Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform], Analytical Chemistry, 2014, 86 (7), 3273–3278.
#Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. [http://www.tandfonline.com/doi/abs/10.1080/07391102.2013.786381?queryID=%24%7BresultBean.queryID%7D#.U72ysvldWv8 Development and Application of Crop Exudate Specific Aptamers], Journal of Biomolecular Structure and Dynamics, 2013, 31 (1), 89.
#McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra43893g#!divAbstract/ Development of a DNA aptamer for direct and selective homocysteine detection in human serum], RSC Advances, 2013, 3 (46), 24415-24422.
#McKeague, M.; DeRosa, M.C. [http://www.hindawi.com/journals/jna/2012/748913/cta/ Challenges and Opportunities for Small Molecule Aptamer Development], Journal of Nucleic Acids, 2012.
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. Database: Journal of Biological Databases and Curation. 2012.   
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. Database: Journal of Biological Databases and Curation. 2012.   
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in Aptamer-Based Biosensors for Food Safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech.
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in Aptamer-Based Biosensors for Food Safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column]. Food Chem. 2011, 127, 1378-1384.
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column]. Food Chem. 2011, 127 (3), 1378-1384.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers]. Int. J. Mol. Sci. 2010, 11, 4864-4881.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers]. Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers]. RNA. 2010, 16, 2252-2262.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers]. RNA. 2010, 16 (11), 2252-2262.


==Useful links==
==Useful links==
*New search for aptamers! [http://aptamerbase.semanticscience.org/?q=base/search/ Aptamer Base]
*New search engine for aptamers! [http://aptamerbase.semanticscience.org/ Aptamer Base]
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance]
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*[[Media:McKeague.pdf|Full curriculum vitae]]

Revision as of 07:52, 6 January 2015

Contact Info

Maureen McKeague

Maureen McKeague
Stanford University
Shriram Center, Room 256
Mail Code 4245
443 Via Ortega
Stanford, CA 94305
Email me through OpenWetWare

I work in the Smolke lab

Education

Research interests

My past, current, and future research aims to improve the workflow of the selection and characterization of small molecule-binding aptamers towards the ultimate goal of developing both in vitro and in vivo biosensors that link important metabolite concentrations to readily detectable signals for high throughput screening and diagnostics.

Publications

  1. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  2. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology, Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.
  3. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A, Toxins, 2014, 6 (8), 2435-2452.
  4. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform, Analytical Chemistry, 2014, 86 (7), 3273–3278.
  5. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and Application of Crop Exudate Specific Aptamers, Journal of Biomolecular Structure and Dynamics, 2013, 31 (1), 89.
  6. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances, 2013, 3 (46), 24415-24422.
  7. McKeague, M.; DeRosa, M.C. Challenges and Opportunities for Small Molecule Aptamer Development, Journal of Nucleic Acids, 2012.
  8. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  9. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in Aptamer-Based Biosensors for Food Safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  10. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column. Food Chem. 2011, 127 (3), 1378-1384.
  11. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers. Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
  12. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers. RNA. 2010, 16 (11), 2252-2262.

Useful links

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Maureen_McKeague&oldid=849317"