User:Maureen McKeague: Difference between revisions

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[[Image:Photo_16.JPG|thumb|right|Maureen McKeague]]
[[Image:Photo_16.JPG|thumb|right|Maureen McKeague]]
'''Maureen McKeague'''
'''Maureen McKeague'''
<br>[http://www.stanford.edu/ Stanford University]
<br>[https://www.ethz.ch/en.html/ ETH Zürich]
<br>Shriram Center, Room 256
<br>LFO D 14
<br> Mail Code 4245
<br>Schmelzbergstrasse 9
<br>443 Via Ortega
<br>8092 Zürich
<br>Stanford, CA 94305
<br>Switzerland
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]
<br>[[Special:Emailuser/Maureen McKeague|Email me through OpenWetWare]]


I work in the [[Smolke]] lab
I work in the [http://www.toxicology.ethz.ch/ Sturla] lab


==Education==
==Education==
<!--Include info about your educational background-->
<!--Include info about your educational background-->
* 2012-2016: NSERC Postdoctoral Fellow in [https://bioengineering.stanford.edu/ Bioengineering],[https://www.stanford.edu/ Stanford University] in the [http://smolkelab.weebly.com/ Smolke] lab
* 2007-2012: PhD in [http://www5.carleton.ca/chemistry/ Chemistry],[http://carleton.ca/ Carleton University] in the [[DeRosa]] lab
* 2007-2012: PhD in [http://www5.carleton.ca/chemistry/ Chemistry],[http://carleton.ca/ Carleton University] in the [[DeRosa]] lab
* 2003-2007: BSc in [http://www1.carleton.ca/biochem/research/biotechnology/ Biochemistry and Biotechnology], [http://carleton.ca/ Carleton University]
* 2003-2007: BSc in [http://www1.carleton.ca/biochem/research/biotechnology/ Biochemistry and Biotechnology], [http://carleton.ca/ Carleton University]


==Research interests==
==Research interests==
I am passionate about improving human health and quality of life using functional nucleic acids. Specifically, my past, current & future research aims to improve the selection and characterization of small molecule-binding aptamers and ribozymes towards the ultimate goal of developing both in vitro and in vivo biosensors for high-throughput drug screening and disease diagnosis.
I have been fascinated by nucleic acids since I learned about the physical basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. Therefore, my past, current, and future research aims to study the unique capabilities of synthetic nucleic acids and use them to solve challenges in health, medicine, and the environment.


==Publications==
==Publications==
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
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#McKeague, M.; Wang, Y-H.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00518 In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control] ACS Chem. Biol, 2015, 10 (11), pp 2463–2467.
#Alssafin, A.; McKeague, M. [http://www.sciencedirect.com/science/article/pii/S0956566317301173 Functional nucleic acids as in vivo metabolite and ion biosensors] Biosensors and Bioelectronics, 2017, 94, 94–106.
#McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. [http://link.springer.com/article/10.1007%2Fs00239-015-9708-6 Analysis of In Vitro Aptamer Selection Parameters]. J Mol Evol. 2015.  
#McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/8/11/336/htm Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A], Toxins, 2016, 8 (11), 336-348.
#Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an01075f#!divAbstract An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers], Analyst 2015, 140, 8, 6643–6651.
#Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. [http://www.cell.com/cell-systems/fulltext/S2405-4712(16)30327-1 Design and Construction of Generalizable RNA-Protein Hybrid Controllers by Level-Matched Genetic Signal Amplification] Cell Systems, 2016, 3 (6), 549–562.
#McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/S1096717616300738 Engineering a Microbial Platform for de novo Biosynthesis of Diverse Methylxanthines] Metabolic Engineering, 2016, 38, 191–203.
#Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. [http://pubs.acs.org/doi/pdf/10.1021/acscombsci.5b00163 Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing]. ACS Combinatorial Science, 2016, 18 (6), 302–313.
#McKeague, M.; Wong, R.S.; Smolke, C.D. [http://nar.oxfordjournals.org/content/early/2016/03/10/nar.gkw151.abstract?keytype=ref&ijkey=P6DDJUKcmpn8XIM Opportunities in the Design and Application of RNA for Gene Expression Control] Nucleic Acids Research, 2016, 44 (7),2987-2999
#McKeague, M.; Wang, Y-H.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00518 In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control] ACS Chemical Biology, 2015, 10 (11), 2463–2467.
#McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. [http://link.springer.com/article/10.1007%2Fs00239-015-9708-6 Analysis of In Vitro Aptamer Selection Parameters]. Journal of Molecular Evolution, 2015,  81(5), 150-161.
#Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an01075f#!divAbstract An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers], Analyst, 2015, 140 (8) 6643–6651.
#McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.[http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b02102 Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation], Analytical Chemistry, 2015, 87 (17), 8608–8612.
#McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.[http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b02102 Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation], Analytical Chemistry, 2015, 87 (17), 8608–8612.
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.  
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile Characterization of Aptamer Kinetic and Equilibrium Binding Properties Using Surface Plasmon Resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.  
#McKeague, M.; DeRosa, M.C. [http://smithandfranklin.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology], Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.  
#McKeague, M.; DeRosa, M.C. [http://smithandfranklin.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology], Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.  
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
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==Useful links==
==Useful links==
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance]
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance] and [http://cen.gext.acs.org/articles/88/i44/Dancing-Chemists-Old-Butter-Asparagus.html/ C&EN]
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*[[Media:CVMCKEAGUE.pdf|Full curriculum vitae]]
*[http://www.oligotherapeutics.org/interview-with-maureen-mckeague-phd/ Interview with the Oligonucleotide Therapeutics Society]
*[http://www.oligotherapeutics.org/interview-with-maureen-mckeague-phd/ Interview with the Oligonucleotide Therapeutics Society]
*[http://aptamersociety.org/about-insoap/ International Society on Aptamers]
*[http://aptamersociety.org/about-insoap/ International Society on Aptamers]
*Building a search engine for aptamers! [http://aptamerbase.semanticscience.org/ Aptamer Base]
*Building a search engine for aptamers! [http://www.freebase.com/base/aptamer Aptamer Base]
*2015 Analytical Chemistry [http://pubs.acs.org/iapps/liveslides/pages/index.htm?mscNo=acs.analchem.5b02102 LiveSlides ]

Revision as of 08:11, 8 March 2017

Contact Info

Maureen McKeague

Maureen McKeague
ETH Zürich
LFO D 14
Schmelzbergstrasse 9
8092 Zürich
Switzerland
Email me through OpenWetWare

I work in the Sturla lab

Education

Research interests

I have been fascinated by nucleic acids since I learned about the physical basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. Therefore, my past, current, and future research aims to study the unique capabilities of synthetic nucleic acids and use them to solve challenges in health, medicine, and the environment.

Publications

  1. Alssafin, A.; McKeague, M. Functional nucleic acids as in vivo metabolite and ion biosensors Biosensors and Bioelectronics, 2017, 94, 94–106.
  2. McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A, Toxins, 2016, 8 (11), 336-348.
  3. Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. Design and Construction of Generalizable RNA-Protein Hybrid Controllers by Level-Matched Genetic Signal Amplification Cell Systems, 2016, 3 (6), 549–562.
  4. McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. Engineering a Microbial Platform for de novo Biosynthesis of Diverse Methylxanthines Metabolic Engineering, 2016, 38, 191–203.
  5. Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing. ACS Combinatorial Science, 2016, 18 (6), 302–313.
  6. McKeague, M.; Wong, R.S.; Smolke, C.D. Opportunities in the Design and Application of RNA for Gene Expression Control Nucleic Acids Research, 2016, 44 (7),2987-2999
  7. McKeague, M.; Wang, Y-H.; Smolke, C.D. In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control ACS Chemical Biology, 2015, 10 (11), 2463–2467.
  8. McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. Analysis of In Vitro Aptamer Selection Parameters. Journal of Molecular Evolution, 2015, 81(5), 150-161.
  9. Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers, Analyst, 2015, 140 (8) 6643–6651.
  10. McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation, Analytical Chemistry, 2015, 87 (17), 8608–8612.
  11. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile Characterization of Aptamer Kinetic and Equilibrium Binding Properties Using Surface Plasmon Resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  12. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology, Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.
  13. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A, Toxins, 2014, 6 (8), 2435-2452.
  14. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform, Analytical Chemistry, 2014, 86 (7), 3273–3278.
  15. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and Application of Crop Exudate Specific Aptamers, Journal of Biomolecular Structure and Dynamics, 2013, 31 (1), 89.
  16. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances, 2013, 3 (46), 24415-24422.
  17. McKeague, M.; DeRosa, M.C. Challenges and Opportunities for Small Molecule Aptamer Development, Journal of Nucleic Acids, 2012.
  18. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  19. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in Aptamer-Based Biosensors for Food Safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  20. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column. Food Chem. 2011, 127 (3), 1378-1384.
  21. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers, Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
  22. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers, RNA. 2010, 16 (11), 2252-2262.

Useful links

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