User:Mauricio Bedoya/Notebook/Project 1/2008/05/03: Difference between revisions
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==Next step ideas== | ==Next step ideas== | ||
* Most important for next experiment : use method to filter out particles with sub-pixel resolution, or use 100x objective with 1.5 mag. | *1. Most important for next experiment : use method to filter out particles with sub-pixel resolution, or use 100x objective with 1.5 mag. | ||
2. Particles stuck to the glass are ok - but this reminds me to double check with you that you are studying the particles at quite a distance from the surface (>10 particle radii at least) to avoid hydrodynamic coupling | *2. Particles stuck to the glass are ok - but this reminds me to double check with you that you are studying the particles at quite a distance from the surface (>10 particle radii at least) to avoid hydrodynamic coupling | ||
3. were there any signs of aggregation? Would it make sense to increase the particle density to get better stats? | *3. were there any signs of aggregation? Would it make sense to increase the particle density to get better stats? | ||
4. Since the difference in alpha is not significant, lets be dramatic and go to a two concentrations above the critical concentration overlap, say 1mg/mL and 1.5 mg/mL and see what things look like there. At these high concentrations - particle motion might be really small, so use 150x magnification. Be sure to get lots of data at these dilutions, since it is a bit expensive. | *4. Since the difference in alpha is not significant, lets be dramatic and go to a two concentrations above the critical concentration overlap, say 1mg/mL and 1.5 mg/mL and see what things look like there. At these high concentrations - particle motion might be really small, so use 150x magnification. Be sure to get lots of data at these dilutions, since it is a bit expensive. | ||
5. Lets also test at a concentration that Heike previous had issues with .75 mg/mL and see if aggregation is occuring - and also to get a nice data point. | *5. Lets also test at a concentration that Heike previous had issues with .75 mg/mL and see if aggregation is occuring - and also to get a nice data point. | ||
6. Lengthscale of movies: if you want to have the long time data, it looks like you'll need more stats - you might as well, so long as you have the samples sitting there and no aggregation is occuring. This also might be good for data for two point MR analysis (at a later date). | *6. Lengthscale of movies: if you want to have the long time data, it looks like you'll need more stats - you might as well, so long as you have the samples sitting there and no aggregation is occuring. This also might be good for data for two point MR analysis (at a later date). | ||
Data Analysis : | *Data Analysis : | ||
1. Can you see any difference in the slope of the two concentrations when you fit it? | *1. Can you see any difference in the slope of the two concentrations when you fit it? | ||
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Revision as of 10:04, 4 May 2008
Microrheology properties of Hyaluronan | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
First HA experiments05/02
05/03
First HA experiments: particle analysis
Next step ideas
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