User:Maximilian Peters

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<!-- Delete this entire line as part of your first edit of your user page --> {{New user}}
 
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==Contact Info==
==Contact Info==
[[Image:OWWEmblem.png|thumb|right|Maximilian Peters (an artistic interpretation)]]
[[Image:OWWEmblem.png|thumb|right|Maximilian Peters (an artistic interpretation)]]
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*Maximilian Peters
*Maximilian Peters
*Hebrew University of Jerusalem
*Hebrew University of Jerusalem
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*Address 1
 
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*Address 2
 
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*City, State, Country etc.
 
*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
-
I work in the [[Your Lab]] at XYZ University.  I learned about [[OpenWetWare]] from google, and I've joined because Learn and contribute.
+
I learned about [[OpenWetWare]] from google, and I've joined because I want to learn and contribute.
-
==Education==
 
-
<!--Include info about your educational background-->
 
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* Year, PhD, Institute
 
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* Year, MS, Institute
 
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* Year, BS, Institute
 
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==Research interests==
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==Site directed mutagenesis protocol==
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<!-- Feel free to add brief descriptions to your research interests as well -->
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http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis
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# Interest 1
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# Interest 2
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==Preparing poly-lysine coated coverslips==
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# Interest 3
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http://openwetware.org/wiki/User:Maximilian_Peters/polylysine
 +
 
 +
==Pharmacology of TRP channels==
 +
[[User:Maximilian_Peters/TRPharmacalogy|List of antagonists and agonists of TRP channels]]
 +
==Useful Links==
 +
===Protein-Protein interaction prediction servers===
 +
http://www.compbio.dundee.ac.uk/www-pips/<br>
 +
http://crdd.osdd.net:8081/ProPrint/submit.jsp<br>
 +
http://www.russell.embl.de/cgi-bin/tools/interprets.pl<br>
==Publications==
==Publications==
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
<biblio>
<biblio>
-
#Paper1 pmid=6947258
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#Paper1 pmid=17478614
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#Paper2 pmid=13718526
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#Paper2 pmid=17712814
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// leave a comment about a paper here
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#Paper3 pmid=17714026
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#Book1 isbn=0879697164
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#Paper4 pmid=19135721
 +
#Paper5 pmid=19535910
</biblio>
</biblio>
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*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]
 +
 +
==Good enyzmes for cutting PCR products close to the end==
 +
AflIII: CCACATGTGG <br>
 +
AscI: GGCGCGCC <br>
 +
AvaI: CCCCCGGGGG <br>
 +
BamHI: CGGGATCCCG <br>
 +
ClaI: CCATCGATGG <br>
 +
EcoRI: GGAATTCC <br>
 +
HaeIII: GGGGCCCC <br>
 +
KpnI: GGGGTACCCC <br>
 +
StuI: AAGGCCTT <br>
 +
XbaI: GCTCTAGAGC <br>
 +
 +
Criteria: >90% digestion in 2h, short sequence<br>
 +
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp
 +
 +
==Solutions and Media==
 +
[[User:Maximilian_Peters/Stock_solutions|List of different stock solutions]]<br><br><br>
 +
Schneiders S2 medium:
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Inorganic salts'''
 +
| align="center" style="background:#f0f0f0;"|'''[g/l]'''
 +
|-
 +
| CaCl2||0.6||
 +
|-
 +
| MgSO4 (anhyd) || 1.807221 ||
 +
|-
 +
| KCl ||01.06.09||
 +
|-
 +
| KH2PO4 || 0.45||
 +
|-
 +
| NaHCO3 || 0.4 ||
 +
|-
 +
| NaCl ||02.01.09||
 +
|-
 +
| Na2HPO4 || 0.7||
 +
|-
 +
| ||||
 +
|-
 +
| Amino acids || [g/l]||
 +
|-
 +
| b-Alanine || 0.5 ||
 +
|-
 +
| L-Arginine || 0.6||
 +
|-
 +
| L-Aspartic acid || 0.4||
 +
|-
 +
| L-Cysteine || 0.06||
 +
|-
 +
| L-Cystine • 2HCl || 0.026732 ||
 +
|-
 +
| L-Glutamic acid || 0.8 ||
 +
|-
 +
| L-Glutamine ||01.08.09||
 +
|-
 +
| Glycine || 0.25 ||
 +
|-
 +
| L-Histidine || 0.4||
 +
|-
 +
| L-Isoleucine || 0.15||
 +
|-
 +
| L-Leucine || 0.15||
 +
|-
 +
| L-Lysine ||01.01.65||
 +
|-
 +
| L-Methionine || 0.15||
 +
|-
 +
| L-Proline ||01.07.09||
 +
|-
 +
| L-Serine || 0.25 ||
 +
|-
 +
| L-Threonine || 0.35||
 +
|-
 +
| L-Tryptophan || 0.1||
 +
|-
 +
| L-Tyrosine 2Na 2H2O || 0.720199||
 +
|-
 +
| L-Valine || 0.3||
 +
|-
 +
| Sugars||||
 +
|-
 +
| D(+)-Glucose ||2||
 +
|-
 +
| D-(+)-Trehalose ||2||
 +
|-
 +
| Other||||
 +
|-
 +
| Fumaric acid || 0.06||
 +
|-
 +
| a-Ketoglutaric acid|| acid ||
 +
|-
 +
| L(-)-Malic acid || 0.6||
 +
|-
 +
| Succinic acid || 0.06||
 +
|-
 +
| Yeast extract ||2||
 +
|-
 +
|
 +
|}
 +
 +
Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf
 +
 +
DMEM - Dulbecco's Modified Eagle's Medium
 +
 +
With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''CHEMICAL NAME'''
 +
| align="center" style="background:#f0f0f0;"|'''Grams per Liter'''
 +
|-
 +
| ||
 +
|-
 +
| CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20||0.26
 +
|-
 +
| FERRIC NITRATE 9-HYDRATE  Fe(NO3)3. 9H2O||0
 +
|-
 +
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0.1
 +
|-
 +
| POTASSIUM CHLORIDE  KCl||0.40
 +
|-
 +
| SODIUM BICARBONATE  NaHCO3||3.70
 +
|-
 +
| SODIUM CHLORIDE  NaCl||6.40
 +
|-
 +
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0.11
 +
|-
 +
| ||
 +
|-
 +
| L-ARGININE, HCl||0.08
 +
|-
 +
| L-CYSTINE 2HCl||0.06
 +
|-
 +
| GLYCINE||0.030
 +
|-
 +
| L-HISTIDINE-HCL-H20||0.04
 +
|-
 +
| L-ISOLEUCINE||0.11
 +
|-
 +
| L-LEUCINE||0.11
 +
|-
 +
| L-LYSINE  HCL||0.15
 +
|-
 +
| L-METHIONINE||0.030
 +
|-
 +
| L-PHENYLALANINE||0.07
 +
|-
 +
| L-SERINE  ||0.04
 +
|-
 +
| L-THREONINE||0.1
 +
|-
 +
| L-TRYPTOPHAN||0.02
 +
|-
 +
| L-TYROSINE,  DISODIUM SALT, DIHYDRATE||0.1
 +
|-
 +
| L-VALINE||0.09
 +
|-
 +
| ||
 +
|-
 +
| CHOLINE CHLORIDE||0
 +
|-
 +
| FOLIC ACID||0
 +
|-
 +
| myo-INOSITOL||0.01
 +
|-
 +
| NIACINAMIDE  ||0
 +
|-
 +
| D-CALCIUM PANTOTHENATE||0
 +
|-
 +
| PYRIDOXINE  HCL||0
 +
|-
 +
| RIBOFLAVIN||0
 +
|-
 +
| THIAMINE  HCL||0
 +
|-
 +
| ||
 +
|-
 +
| DEXTROSE C6H1206||1.00
 +
|-
 +
| PHENOL RED Na||0.02
 +
|-
 +
| SODIUM PYRUVATE||0.11
 +
|-
 +
|
 +
|}
 +
 +
Reference: http://www.cellsoup.com/cmandrfr.html
 +
 +
 +
<b>RPMI 1640</b>
 +
with Sodium Bicarbonate, without L- Glutamine,
 +
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''CHEMICAL NAME'''
 +
| align="center" style="background:#f0f0f0;"|'''Grams per Liter'''
 +
|-
 +
| ||
 +
|-
 +
| CALCIUM NITRATE Ca(NO3)2 . 4H2O||0.10
 +
|-
 +
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0.05
 +
|-
 +
| POTASSIUM CHLORIDE  KCl||0.40
 +
|-
 +
| SODIUM CHLORIDE  NaCl||6.00
 +
|-
 +
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0.80
 +
|-
 +
| ||
 +
|-
 +
| L-ARGININE  ||0.20
 +
|-
 +
| L-ASPARAGINE||0.05
 +
|-
 +
| L-ASPARTIC ACID||0.020
 +
|-
 +
| L-CYSTINE 2HCl||0.07
 +
|-
 +
| L-GLUTAMIC ACID||0.02
 +
|-
 +
| GLYCINE||0.01
 +
|-
 +
| L-HISTIDINE||0.02
 +
|-
 +
| HYDROXY-L-PROLINE  ||0.020
 +
|-
 +
| L-ISOLEUCINE||0.05
 +
|-
 +
| L-LEUCINE||0.05
 +
|-
 +
| L-LYSINE  HCL||0.04
 +
|-
 +
| L-METHIONINE||0.02
 +
|-
 +
| L-PHENYLALANINE||0.02
 +
|-
 +
| L-PROLINE  ||0.02
 +
|-
 +
| L-SERINE  ||0.03
 +
|-
 +
| L-THREONINE||0.02
 +
|-
 +
| L-TRYPTOPHAN||0.01
 +
|-
 +
| L-TYROSINE.  DISODIUM SALT, DIHYDRATE||0.03
 +
|-
 +
| L-VALINE||0.02
 +
|-
 +
| ||
 +
|-
 +
| D-BIOTIN  ||0
 +
|-
 +
| CHOLINE CHLORIDE||0
 +
|-
 +
| FOLIC ACID||0
 +
|-
 +
| myo-INOSITOL||0.04
 +
|-
 +
| NIACINAMIDE  ||0
 +
|-
 +
| P-AMINOBENZOIC ACID  ||0
 +
|-
 +
| D-CALCIUM PANTOTHENATE||0
 +
|-
 +
| PYRIDOXINE  HCL||0
 +
|-
 +
| RIBOFLAVIN||0.0002
 +
|-
 +
| THIAMINE  HCL||0
 +
|-
 +
| CYANOCOBALAMIN  VITAMIN  B12||0
 +
|-
 +
| ||
 +
|-
 +
| DEXTROSE C6H1206||2.00
 +
|-
 +
| GLUTATHIONE  (REDUCED)||0
 +
|-
 +
| PHENOL RED Na||0.01
 +
|-
 +
|
 +
|}
 +
 +
Reference: http://www.cellsoup.com/cmandrfr.html

Current revision

Contents

Contact Info

Maximilian Peters (an artistic interpretation)
Maximilian Peters (an artistic interpretation)

I learned about OpenWetWare from google, and I've joined because I want to learn and contribute.


Site directed mutagenesis protocol

http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis

Preparing poly-lysine coated coverslips

http://openwetware.org/wiki/User:Maximilian_Peters/polylysine

Pharmacology of TRP channels

List of antagonists and agonists of TRP channels

Useful Links

Protein-Protein interaction prediction servers

http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

  1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. . pmid:17478614. PubMed HubMed [Paper1]
  2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. . pmid:17712814. PubMed HubMed [Paper2]
  3. Peters M and Kogan NM. . pmid:17714026. PubMed HubMed [Paper3]
  4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. . pmid:19135721. PubMed HubMed [Paper4]
  5. Parnas M, Peters M, and Minke B. . pmid:19535910. PubMed HubMed [Paper5]
All Medline abstracts: PubMed HubMed

Useful links

Good enyzmes for cutting PCR products close to the end

AflIII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media

List of different stock solutions


Schneiders S2 medium:

Inorganic salts [g/l]
CaCl20.6
MgSO4 (anhyd) 1.807221
KCl 01.06.09
KH2PO4 0.45
NaHCO3 0.4
NaCl 02.01.09
Na2HPO4 0.7
Amino acids [g/l]
b-Alanine 0.5
L-Arginine 0.6
L-Aspartic acid 0.4
L-Cysteine 0.06
L-Cystine • 2HCl 0.026732
L-Glutamic acid 0.8
L-Glutamine 01.08.09
Glycine 0.25
L-Histidine 0.4
L-Isoleucine 0.15
L-Leucine 0.15
L-Lysine 01.01.65
L-Methionine 0.15
L-Proline 01.07.09
L-Serine 0.25
L-Threonine 0.35
L-Tryptophan 0.1
L-Tyrosine 2Na 2H2O 0.720199
L-Valine 0.3
Sugars
D(+)-Glucose 2
D-(+)-Trehalose 2
Other
Fumaric acid 0.06
a-Ketoglutaric acid acid
L(-)-Malic acid 0.6
Succinic acid 0.06
Yeast extract 2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H200.26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O0
MAGNESIUM SULFATE ANHYDROUS MgSO40.1
POTASSIUM CHLORIDE KCl0.40
SODIUM BICARBONATE NaHCO33.70
SODIUM CHLORIDE NaCl6.40
SODIUM PHOSPHATE DIBASIC Na2HPO40.11
L-ARGININE, HCl0.08
L-CYSTINE 2HCl0.06
GLYCINE0.030
L-HISTIDINE-HCL-H200.04
L-ISOLEUCINE0.11
L-LEUCINE0.11
L-LYSINE HCL0.15
L-METHIONINE0.030
L-PHENYLALANINE0.07
L-SERINE 0.04
L-THREONINE0.1
L-TRYPTOPHAN0.02
L-TYROSINE, DISODIUM SALT, DIHYDRATE0.1
L-VALINE0.09
CHOLINE CHLORIDE0
FOLIC ACID0
myo-INOSITOL0.01
NIACINAMIDE 0
D-CALCIUM PANTOTHENATE0
PYRIDOXINE HCL0
RIBOFLAVIN0
THIAMINE HCL0
DEXTROSE C6H12061.00
PHENOL RED Na0.02
SODIUM PYRUVATE0.11

Reference: http://www.cellsoup.com/cmandrfr.html


RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,


CHEMICAL NAME Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O0.10
MAGNESIUM SULFATE ANHYDROUS MgSO40.05
POTASSIUM CHLORIDE KCl0.40
SODIUM CHLORIDE NaCl6.00
SODIUM PHOSPHATE DIBASIC Na2HPO40.80
L-ARGININE 0.20
L-ASPARAGINE0.05
L-ASPARTIC ACID0.020
L-CYSTINE 2HCl0.07
L-GLUTAMIC ACID0.02
GLYCINE0.01
L-HISTIDINE0.02
HYDROXY-L-PROLINE 0.020
L-ISOLEUCINE0.05
L-LEUCINE0.05
L-LYSINE HCL0.04
L-METHIONINE0.02
L-PHENYLALANINE0.02
L-PROLINE 0.02
L-SERINE 0.03
L-THREONINE0.02
L-TRYPTOPHAN0.01
L-TYROSINE. DISODIUM SALT, DIHYDRATE0.03
L-VALINE0.02
D-BIOTIN 0
CHOLINE CHLORIDE0
FOLIC ACID0
myo-INOSITOL0.04
NIACINAMIDE 0
P-AMINOBENZOIC ACID 0
D-CALCIUM PANTOTHENATE0
PYRIDOXINE HCL0
RIBOFLAVIN0.0002
THIAMINE HCL0
CYANOCOBALAMIN VITAMIN B120
DEXTROSE C6H12062.00
GLUTATHIONE (REDUCED)0
PHENOL RED Na0.01

Reference: http://www.cellsoup.com/cmandrfr.html

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