User:Maximilian Peters: Difference between revisions
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*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]] | *[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]] | ||
I learned about [[OpenWetWare]] from google, and I've joined because | I learned about [[OpenWetWare]] from google, and I've joined because I want to learn and contribute. | ||
==Site directed mutagenesis protocol== | |||
http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis | |||
==Preparing poly-lysine coated coverslips== | |||
http://openwetware.org/wiki/User:Maximilian_Peters/polylysine | |||
==Pharmacology of TRP channels== | |||
[[User:Maximilian_Peters/TRPharmacalogy|List of antagonists and agonists of TRP channels]] | |||
==Useful Links== | |||
===Protein-Protein interaction prediction servers=== | |||
http://www.compbio.dundee.ac.uk/www-pips/<br> | |||
http://crdd.osdd.net:8081/ProPrint/submit.jsp<br> | |||
http://www.russell.embl.de/cgi-bin/tools/interprets.pl<br> | |||
==Publications== | ==Publications== | ||
Line 22: | Line 37: | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
==Good enyzmes for cutting PCR products close to the end== | |||
AflIII: CCACATGTGG <br> | |||
AscI: GGCGCGCC <br> | |||
AvaI: CCCCCGGGGG <br> | |||
BamHI: CGGGATCCCG <br> | |||
ClaI: CCATCGATGG <br> | |||
EcoRI: GGAATTCC <br> | |||
HaeIII: GGGGCCCC <br> | |||
KpnI: GGGGTACCCC <br> | |||
StuI: AAGGCCTT <br> | |||
XbaI: GCTCTAGAGC <br> | |||
Criteria: >90% digestion in 2h, short sequence<br> | |||
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp | |||
==Solutions and Media== | ==Solutions and Media== | ||
[[User:Maximilian_Peters/Stock_solutions|List of different stock solutions]]<br><br><br> | |||
Schneiders S2 medium: | Schneiders S2 medium: | ||
Line 206: | Line 235: | ||
| || | | || | ||
|- | |- | ||
| CALCIUM NITRATE Ca(NO3)2 . 4H2O||0 | | CALCIUM NITRATE Ca(NO3)2 . 4H2O||0.10 | ||
|- | |- | ||
| MAGNESIUM SULFATE ANHYDROUS MgSO4||0 | | MAGNESIUM SULFATE ANHYDROUS MgSO4||0.05 | ||
|- | |- | ||
| POTASSIUM CHLORIDE KCl||0 | | POTASSIUM CHLORIDE KCl||0.40 | ||
|- | |- | ||
| SODIUM CHLORIDE NaCl||6 | | SODIUM CHLORIDE NaCl||6.00 | ||
|- | |- | ||
| SODIUM PHOSPHATE DIBASIC Na2HPO4||0 | | SODIUM PHOSPHATE DIBASIC Na2HPO4||0.80 | ||
|- | |- | ||
| || | | || | ||
|- | |- | ||
| L-ARGININE ||0 | | L-ARGININE ||0.20 | ||
|- | |- | ||
| L-ASPARAGINE||0 | | L-ASPARAGINE||0.05 | ||
|- | |- | ||
| L-ASPARTIC ACID||0 | | L-ASPARTIC ACID||0.020 | ||
|- | |- | ||
| L-CYSTINE 2HCl||0 | | L-CYSTINE 2HCl||0.07 | ||
|- | |- | ||
| L-GLUTAMIC ACID||0 | | L-GLUTAMIC ACID||0.02 | ||
|- | |- | ||
| GLYCINE||0 | | GLYCINE||0.01 | ||
|- | |- | ||
| L-HISTIDINE||0 | | L-HISTIDINE||0.02 | ||
|- | |- | ||
| HYDROXY-L-PROLINE ||0 | | HYDROXY-L-PROLINE ||0.020 | ||
|- | |- | ||
| L-ISOLEUCINE||0 | | L-ISOLEUCINE||0.05 | ||
|- | |- | ||
| L-LEUCINE||0 | | L-LEUCINE||0.05 | ||
|- | |- | ||
| L-LYSINE HCL||0 | | L-LYSINE HCL||0.04 | ||
|- | |- | ||
| L-METHIONINE||0 | | L-METHIONINE||0.02 | ||
|- | |- | ||
| L-PHENYLALANINE||0 | | L-PHENYLALANINE||0.02 | ||
|- | |- | ||
| L-PROLINE ||0 | | L-PROLINE ||0.02 | ||
|- | |- | ||
| L-SERINE ||0 | | L-SERINE ||0.03 | ||
|- | |- | ||
| L-THREONINE||0 | | L-THREONINE||0.02 | ||
|- | |- | ||
| L-TRYPTOPHAN||0 | | L-TRYPTOPHAN||0.01 | ||
|- | |- | ||
| L-TYROSINE | | L-TYROSINE. DISODIUM SALT, DIHYDRATE||0.03 | ||
|- | |- | ||
| L-VALINE||0 | | L-VALINE||0.02 | ||
|- | |- | ||
| || | | || | ||
Line 264: | Line 293: | ||
| FOLIC ACID||0 | | FOLIC ACID||0 | ||
|- | |- | ||
| myo-INOSITOL||0 | | myo-INOSITOL||0.04 | ||
|- | |- | ||
| NIACINAMIDE ||0 | | NIACINAMIDE ||0 | ||
Line 274: | Line 303: | ||
| PYRIDOXINE HCL||0 | | PYRIDOXINE HCL||0 | ||
|- | |- | ||
| RIBOFLAVIN||0 | | RIBOFLAVIN||0.0002 | ||
|- | |- | ||
| THIAMINE HCL||0 | | THIAMINE HCL||0 | ||
Line 282: | Line 311: | ||
| || | | || | ||
|- | |- | ||
| DEXTROSE C6H1206||2 | | DEXTROSE C6H1206||2.00 | ||
|- | |- | ||
| GLUTATHIONE (REDUCED)||0 | | GLUTATHIONE (REDUCED)||0 | ||
|- | |- | ||
| PHENOL RED Na||0 | | PHENOL RED Na||0.01 | ||
|- | |- | ||
| | | |
Latest revision as of 06:24, 18 July 2012
Contact Info
- Maximilian Peters
- Hebrew University of Jerusalem
- Email me through OpenWetWare
I learned about OpenWetWare from google, and I've joined because I want to learn and contribute.
Site directed mutagenesis protocol
http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis
Preparing poly-lysine coated coverslips
http://openwetware.org/wiki/User:Maximilian_Peters/polylysine
Pharmacology of TRP channels
List of antagonists and agonists of TRP channels
Useful Links
Protein-Protein interaction prediction servers
http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl
Publications
- Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. A cannabinoid anticancer quinone, HU-331, is more potent and less cardiotoxic than doxorubicin: a comparative in vivo study. J Pharmacol Exp Ther. 2007 Aug;322(2):646-53. DOI:10.1124/jpet.107.120865 |
- Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. Cannabidiol--recent advances. Chem Biodivers. 2007 Aug;4(8):1678-92. DOI:10.1002/cbdv.200790147 |
- Peters M and Kogan NM. HU-331: a cannabinoid quinone, with uncommon cytotoxic properties and low toxicity. Expert Opin Investig Drugs. 2007 Sep;16(9):1405-13. DOI:10.1517/13543784.16.9.1405 |
- Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels. Cell Calcium. 2009 Mar;45(3):300-9. DOI:10.1016/j.ceca.2008.11.009 |
- Parnas M, Peters M, and Minke B. Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action. Channels (Austin). 2009 May-Jun;3(3):164-6. DOI:10.4161/chan.3.3.8873 |
Useful links
Good enyzmes for cutting PCR products close to the end
AflIII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC
Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp
Solutions and Media
List of different stock solutions
Schneiders S2 medium:
Inorganic salts | [g/l] | |
CaCl2 | 0.6 | |
MgSO4 (anhyd) | 1.807221 | |
KCl | 01.06.09 | |
KH2PO4 | 0.45 | |
NaHCO3 | 0.4 | |
NaCl | 02.01.09 | |
Na2HPO4 | 0.7 | |
Amino acids | [g/l] | |
b-Alanine | 0.5 | |
L-Arginine | 0.6 | |
L-Aspartic acid | 0.4 | |
L-Cysteine | 0.06 | |
L-Cystine • 2HCl | 0.026732 | |
L-Glutamic acid | 0.8 | |
L-Glutamine | 01.08.09 | |
Glycine | 0.25 | |
L-Histidine | 0.4 | |
L-Isoleucine | 0.15 | |
L-Leucine | 0.15 | |
L-Lysine | 01.01.65 | |
L-Methionine | 0.15 | |
L-Proline | 01.07.09 | |
L-Serine | 0.25 | |
L-Threonine | 0.35 | |
L-Tryptophan | 0.1 | |
L-Tyrosine 2Na 2H2O | 0.720199 | |
L-Valine | 0.3 | |
Sugars | ||
D(+)-Glucose | 2 | |
D-(+)-Trehalose | 2 | |
Other | ||
Fumaric acid | 0.06 | |
a-Ketoglutaric acid | acid | |
L(-)-Malic acid | 0.6 | |
Succinic acid | 0.06 | |
Yeast extract | 2 | |
DMEM - Dulbecco's Modified Eagle's Medium
With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine
CHEMICAL NAME | Grams per Liter |
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20 | 0.26 |
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O | 0 |
MAGNESIUM SULFATE ANHYDROUS MgSO4 | 0.1 |
POTASSIUM CHLORIDE KCl | 0.40 |
SODIUM BICARBONATE NaHCO3 | 3.70 |
SODIUM CHLORIDE NaCl | 6.40 |
SODIUM PHOSPHATE DIBASIC Na2HPO4 | 0.11 |
L-ARGININE, HCl | 0.08 |
L-CYSTINE 2HCl | 0.06 |
GLYCINE | 0.030 |
L-HISTIDINE-HCL-H20 | 0.04 |
L-ISOLEUCINE | 0.11 |
L-LEUCINE | 0.11 |
L-LYSINE HCL | 0.15 |
L-METHIONINE | 0.030 |
L-PHENYLALANINE | 0.07 |
L-SERINE | 0.04 |
L-THREONINE | 0.1 |
L-TRYPTOPHAN | 0.02 |
L-TYROSINE, DISODIUM SALT, DIHYDRATE | 0.1 |
L-VALINE | 0.09 |
CHOLINE CHLORIDE | 0 |
FOLIC ACID | 0 |
myo-INOSITOL | 0.01 |
NIACINAMIDE | 0 |
D-CALCIUM PANTOTHENATE | 0 |
PYRIDOXINE HCL | 0 |
RIBOFLAVIN | 0 |
THIAMINE HCL | 0 |
DEXTROSE C6H1206 | 1.00 |
PHENOL RED Na | 0.02 |
SODIUM PYRUVATE | 0.11 |
Reference: http://www.cellsoup.com/cmandrfr.html
RPMI 1640
with Sodium Bicarbonate, without L- Glutamine,
CHEMICAL NAME | Grams per Liter |
CALCIUM NITRATE Ca(NO3)2 . 4H2O | 0.10 |
MAGNESIUM SULFATE ANHYDROUS MgSO4 | 0.05 |
POTASSIUM CHLORIDE KCl | 0.40 |
SODIUM CHLORIDE NaCl | 6.00 |
SODIUM PHOSPHATE DIBASIC Na2HPO4 | 0.80 |
L-ARGININE | 0.20 |
L-ASPARAGINE | 0.05 |
L-ASPARTIC ACID | 0.020 |
L-CYSTINE 2HCl | 0.07 |
L-GLUTAMIC ACID | 0.02 |
GLYCINE | 0.01 |
L-HISTIDINE | 0.02 |
HYDROXY-L-PROLINE | 0.020 |
L-ISOLEUCINE | 0.05 |
L-LEUCINE | 0.05 |
L-LYSINE HCL | 0.04 |
L-METHIONINE | 0.02 |
L-PHENYLALANINE | 0.02 |
L-PROLINE | 0.02 |
L-SERINE | 0.03 |
L-THREONINE | 0.02 |
L-TRYPTOPHAN | 0.01 |
L-TYROSINE. DISODIUM SALT, DIHYDRATE | 0.03 |
L-VALINE | 0.02 |
D-BIOTIN | 0 |
CHOLINE CHLORIDE | 0 |
FOLIC ACID | 0 |
myo-INOSITOL | 0.04 |
NIACINAMIDE | 0 |
P-AMINOBENZOIC ACID | 0 |
D-CALCIUM PANTOTHENATE | 0 |
PYRIDOXINE HCL | 0 |
RIBOFLAVIN | 0.0002 |
THIAMINE HCL | 0 |
CYANOCOBALAMIN VITAMIN B12 | 0 |
DEXTROSE C6H1206 | 2.00 |
GLUTATHIONE (REDUCED) | 0 |
PHENOL RED Na | 0.01 |
Reference: http://www.cellsoup.com/cmandrfr.html