User:Maximilian Peters

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(Solutions and Media)
(Solutions and Media)
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==Solutions and Media==
==Solutions and Media==
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[[Maximilian_Peters/Stock solutions|Stock solutions]]
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[[User:Maximilian_Peters/Stock_solutions|List of different stock solutions]]<br><br><br>
Schneiders S2 medium:
Schneiders S2 medium:

Revision as of 11:59, 30 November 2009

Contents

Contact Info

Maximilian Peters (an artistic interpretation)
Maximilian Peters (an artistic interpretation)

I learned about OpenWetWare from google, and I've joined because Learn and contribute.

Protocols

Useful Links

Protein-Protein interaction prediction servers

http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

  1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. . pmid:17478614. PubMed HubMed [Paper1]
  2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. . pmid:17712814. PubMed HubMed [Paper2]
  3. Peters M and Kogan NM. . pmid:17714026. PubMed HubMed [Paper3]
  4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. . pmid:19135721. PubMed HubMed [Paper4]
  5. Parnas M, Peters M, and Minke B. . pmid:19535910. PubMed HubMed [Paper5]
All Medline abstracts: PubMed HubMed

Useful links

Good enyzmes for cutting PCR products close to the end

AflII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media

List of different stock solutions


Schneiders S2 medium:

Inorganic salts [g/l]
CaCl20.6
MgSO4 (anhyd) 1.807221
KCl 01.06.09
KH2PO4 0.45
NaHCO3 0.4
NaCl 02.01.09
Na2HPO4 0.7
Amino acids [g/l]
b-Alanine 0.5
L-Arginine 0.6
L-Aspartic acid 0.4
L-Cysteine 0.06
L-Cystine • 2HCl 0.026732
L-Glutamic acid 0.8
L-Glutamine 01.08.09
Glycine 0.25
L-Histidine 0.4
L-Isoleucine 0.15
L-Leucine 0.15
L-Lysine 01.01.65
L-Methionine 0.15
L-Proline 01.07.09
L-Serine 0.25
L-Threonine 0.35
L-Tryptophan 0.1
L-Tyrosine 2Na 2H2O 0.720199
L-Valine 0.3
Sugars
D(+)-Glucose 2
D-(+)-Trehalose 2
Other
Fumaric acid 0.06
a-Ketoglutaric acid acid
L(-)-Malic acid 0.6
Succinic acid 0.06
Yeast extract 2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H200.26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O0
MAGNESIUM SULFATE ANHYDROUS MgSO40.1
POTASSIUM CHLORIDE KCl0.40
SODIUM BICARBONATE NaHCO33.70
SODIUM CHLORIDE NaCl6.40
SODIUM PHOSPHATE DIBASIC Na2HPO40.11
L-ARGININE, HCl0.08
L-CYSTINE 2HCl0.06
GLYCINE0.030
L-HISTIDINE-HCL-H200.04
L-ISOLEUCINE0.11
L-LEUCINE0.11
L-LYSINE HCL0.15
L-METHIONINE0.030
L-PHENYLALANINE0.07
L-SERINE 0.04
L-THREONINE0.1
L-TRYPTOPHAN0.02
L-TYROSINE, DISODIUM SALT, DIHYDRATE0.1
L-VALINE0.09
CHOLINE CHLORIDE0
FOLIC ACID0
myo-INOSITOL0.01
NIACINAMIDE 0
D-CALCIUM PANTOTHENATE0
PYRIDOXINE HCL0
RIBOFLAVIN0
THIAMINE HCL0
DEXTROSE C6H12061.00
PHENOL RED Na0.02
SODIUM PYRUVATE0.11

Reference: http://www.cellsoup.com/cmandrfr.html


RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,


CHEMICAL NAME Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O0.10
MAGNESIUM SULFATE ANHYDROUS MgSO40.05
POTASSIUM CHLORIDE KCl0.40
SODIUM CHLORIDE NaCl6.00
SODIUM PHOSPHATE DIBASIC Na2HPO40.80
L-ARGININE 0.20
L-ASPARAGINE0.05
L-ASPARTIC ACID0.020
L-CYSTINE 2HCl0.07
L-GLUTAMIC ACID0.02
GLYCINE0.01
L-HISTIDINE0.02
HYDROXY-L-PROLINE 0.020
L-ISOLEUCINE0.05
L-LEUCINE0.05
L-LYSINE HCL0.04
L-METHIONINE0.02
L-PHENYLALANINE0.02
L-PROLINE 0.02
L-SERINE 0.03
L-THREONINE0.02
L-TRYPTOPHAN0.01
L-TYROSINE. DISODIUM SALT, DIHYDRATE0.03
L-VALINE0.02
D-BIOTIN 0
CHOLINE CHLORIDE0
FOLIC ACID0
myo-INOSITOL0.04
NIACINAMIDE 0
P-AMINOBENZOIC ACID 0
D-CALCIUM PANTOTHENATE0
PYRIDOXINE HCL0
RIBOFLAVIN0.0002
THIAMINE HCL0
CYANOCOBALAMIN VITAMIN B120
DEXTROSE C6H12062.00
GLUTATHIONE (REDUCED)0
PHENOL RED Na0.01

Reference: http://www.cellsoup.com/cmandrfr.html

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