User:Maximilian Peters

Contact Info

Maximilian Peters (an artistic interpretation)

• Maximilian Peters
• Hebrew University of Jerusalem
• Email me through OpenWetWare

I learned about OpenWetWare from google, and I've joined because Learn and contribute.

Site directed mutagenesis protocol

http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis

Pharmacology of TRP channels

List of antagonists and agonists of TRP channels

Useful Links

Protein-Protein interaction prediction servers

http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. A cannabinoid anticancer quinone, HU-331, is more potent and less cardiotoxic than doxorubicin: a comparative in vivo study. J Pharmacol Exp Ther. 2007 Aug;322(2):646-53. DOI:10.1124/jpet.107.120865 |
2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. Cannabidiol--recent advances. Chem Biodivers. 2007 Aug;4(8):1678-92. DOI:10.1002/cbdv.200790147 |
3. Peters M and Kogan NM. HU-331: a cannabinoid quinone, with uncommon cytotoxic properties and low toxicity. Expert Opin Investig Drugs. 2007 Sep;16(9):1405-13. DOI:10.1517/13543784.16.9.1405 |
4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels. Cell Calcium. 2009 Mar;45(3):300-9. DOI:10.1016/j.ceca.2008.11.009 |
5. Parnas M, Peters M, and Minke B. Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action. Channels (Austin). 2009 May-Jun;3(3):164-6. DOI:10.4161/chan.3.3.8873 |

Useful links

• Introductory tutorial
• OpenWetWare help pages

Good enyzmes for cutting PCR products close to the end

AflIII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media

List of different stock solutions


Schneiders S2 medium:

Inorganic salts	[g/l]
CaCl2	0.6
MgSO4 (anhyd)	1.807221
KCl	01.06.09
KH2PO4	0.45
NaHCO3	0.4
NaCl	02.01.09
Na2HPO4	0.7
Amino acids	[g/l]
b-Alanine	0.5
L-Arginine	0.6
L-Aspartic acid	0.4
L-Cysteine	0.06
L-Cystine • 2HCl	0.026732
L-Glutamic acid	0.8
L-Glutamine	01.08.09
Glycine	0.25
L-Histidine	0.4
L-Isoleucine	0.15
L-Leucine	0.15
L-Lysine	01.01.65
L-Methionine	0.15
L-Proline	01.07.09
L-Serine	0.25
L-Threonine	0.35
L-Tryptophan	0.1
L-Tyrosine 2Na 2H2O	0.720199
L-Valine	0.3
Sugars
D(+)-Glucose	2
D-(+)-Trehalose	2
Other
Fumaric acid	0.06
a-Ketoglutaric acid	acid
L(-)-Malic acid	0.6
Succinic acid	0.06
Yeast extract	2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME	Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20	0.26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O	0
MAGNESIUM SULFATE ANHYDROUS MgSO4	0.1
POTASSIUM CHLORIDE KCl	0.40
SODIUM BICARBONATE NaHCO3	3.70
SODIUM CHLORIDE NaCl	6.40
SODIUM PHOSPHATE DIBASIC Na2HPO4	0.11
L-ARGININE, HCl	0.08
L-CYSTINE 2HCl	0.06
GLYCINE	0.030
L-HISTIDINE-HCL-H20	0.04
L-ISOLEUCINE	0.11
L-LEUCINE	0.11
L-LYSINE HCL	0.15
L-METHIONINE	0.030
L-PHENYLALANINE	0.07
L-SERINE	0.04
L-THREONINE	0.1
L-TRYPTOPHAN	0.02
L-TYROSINE, DISODIUM SALT, DIHYDRATE	0.1
L-VALINE	0.09
CHOLINE CHLORIDE	0
FOLIC ACID	0
myo-INOSITOL	0.01
NIACINAMIDE	0
D-CALCIUM PANTOTHENATE	0
PYRIDOXINE HCL	0
RIBOFLAVIN	0
THIAMINE HCL	0
DEXTROSE C6H1206	1.00
PHENOL RED Na	0.02
SODIUM PYRUVATE	0.11

Reference: http://www.cellsoup.com/cmandrfr.html

RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,

CHEMICAL NAME	Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O	0.10
MAGNESIUM SULFATE ANHYDROUS MgSO4	0.05
POTASSIUM CHLORIDE KCl	0.40
SODIUM CHLORIDE NaCl	6.00
SODIUM PHOSPHATE DIBASIC Na2HPO4	0.80
L-ARGININE	0.20
L-ASPARAGINE	0.05
L-ASPARTIC ACID	0.020
L-CYSTINE 2HCl	0.07
L-GLUTAMIC ACID	0.02
GLYCINE	0.01
L-HISTIDINE	0.02
HYDROXY-L-PROLINE	0.020
L-ISOLEUCINE	0.05
L-LEUCINE	0.05
L-LYSINE HCL	0.04
L-METHIONINE	0.02
L-PHENYLALANINE	0.02
L-PROLINE	0.02
L-SERINE	0.03
L-THREONINE	0.02
L-TRYPTOPHAN	0.01
L-TYROSINE. DISODIUM SALT, DIHYDRATE	0.03
L-VALINE	0.02
D-BIOTIN	0
CHOLINE CHLORIDE	0
FOLIC ACID	0
myo-INOSITOL	0.04
NIACINAMIDE	0
P-AMINOBENZOIC ACID	0
D-CALCIUM PANTOTHENATE	0
PYRIDOXINE HCL	0
RIBOFLAVIN	0.0002
THIAMINE HCL	0
CYANOCOBALAMIN VITAMIN B12	0
DEXTROSE C6H1206	2.00
GLUTATHIONE (REDUCED)	0
PHENOL RED Na	0.01

Reference: http://www.cellsoup.com/cmandrfr.html