User:Maximilian Peters/Stock solutions

Bradykinin

Molecular weight: 1060.21 g/mol
Concentration stock solution: 0.5 mM, 1 mg per 1.8 mL H₂O
Usual concentration for experiments: 0.1 μM
Stability: 3-6 months
Storage: -20°C
Suppliers:
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate

Cytosine arabinoside

Molecular weight: 279.68 g/mol
Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: C6645

Notes: 300 μl aliquots

DNAse

Concentration stock solution: 1 mg per 50 μL H₂O
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 50 μl aliquots

Glutamine

Molecular weight: 146.14 g/mol
Concentration stock solution: 0.2 M, 29.228 g per 1000 mL
Usual concentration for experiments:
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: 1 ml aliquots

Insulin

Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl
Usual concentration for experiments: μM
Stability: ?
Storage: -20°C
Suppliers: Sigma, I5500
Incompatibilities:
Notes: 200 μl aliqouts

Lanthanum chloride

Molecular weight: 371.37 g/mol (*7H₂O)
Concentration stock solution: 0.5M, 186 mg per 1000 mL H₂O
Usual concentration for experiments: mM to μM
Stability: >3 months
Storage: +4°C
Suppliers:
Incompatibilities: precipitates with carbonates (buffer!), EGTA and BAPTA have higher affinity to La³⁺ than to Ca²⁺
Notes:

Nifedepine

Molecular weight: 346.335 g/mol
Concentration stock solution: 25 mM, 8.66 mg per 1000 μL DMSO
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Notes: N7634 Sigma, very light sensitive, protect solutions from excessive light, preferable fresh stock each day

Probenicid

Molecular weight: 285.36 g/mol
Concentration stock solution: 250 mM, 71.25 mg per 1 mL of buffer (e.g. HBBS)
Usual concentration for experiments: 1-2.5 mM
Stability: <= 6 months
Storage: -20°C
Suppliers: Sigma, Invitrogen, ABCR
Incompatibilities:
Notes: Invitrogen sells Probenicid (water soluble) which seems to be (according to molecular weight) simply the sodium salt
http://probes.invitrogen.com/media/pis/mp36400.pdf
Dissolve the poweder in 90-95% of the calculated amount of buffer and add 10M NaOH to the desired volume

SKF-96365

Molecular weight:
Concentration stock solution: 50 mM in H₂O, 20.15 mg per 1000 μL
Usual concentration for experiments: 1-50 μM
Stability: at least 1 month
Storage: -20°C
Suppliers:
Notes:

Trypsin

Concentration stock solution: 10 mg/200 μL H₂O
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 200 μL aliquots

Template

Molecular weight: g/mol
Concentration stock solution: mM, 1 mg per 1.8 mL
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: