User:Maximilian Peters/Stock solutions

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==Template==
 
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Molecular weight: g/mol<br>
 
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Concentration stock solution: mM, 1 mg per 1.8 mL  <br>
 
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Usual concentration for experiments: μM<br>
 
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Stability: 2-4 weeks <br>
 
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Storage: -20°C <br>
 
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Suppliers: <br>
 
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Incompatibilities: <br>
 
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Notes: <br><br>
 
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==Bradykinin==
==Bradykinin==
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Suppliers: <br>
Suppliers: <br>
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate<br><br>
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate<br><br>
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==Cytosine arabinoside==
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Molecular weight: 279.68 g/mol<br>
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Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml <br>
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Stability: ? <br>
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Storage: -20°C <br>
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Suppliers: Sigma<br>
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Incompatibilities: <br>
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Notes: C6645<br><br>
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Notes: 300 μl aliquots<br><br>
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==DNAse==
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Concentration stock solution: 1 mg per 50 μL H<sub>2</sub>O<br>
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Stability: 2-4 weeks <br>
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Storage: -20°C <br>
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Suppliers: <br>
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Incompatibilities: <br>
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Notes: 50 μl aliquots <br><br>
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==Glutamine==
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Molecular weight: 146.14 g/mol<br>
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Concentration stock solution: 0.2 M, 29.228 g per 1000 mL  <br>
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Usual concentration for experiments: <br>
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Stability: ? <br>
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Storage: -20°C <br>
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Suppliers: Sigma<br>
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Incompatibilities: <br>
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Notes: 1 ml aliquots<br><br>
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==Insulin==
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Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl <br>
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Usual concentration for experiments: μM<br>
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Stability: ? <br>
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Storage: -20°C <br>
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Suppliers: Sigma, I5500<br>
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Incompatibilities: <br>
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Notes: 200 μl aliqouts <br><br>
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==Lanthanum chloride==
==Lanthanum chloride==
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Storage: -20°C <br>
Storage: -20°C <br>
Suppliers: <br>
Suppliers: <br>
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Notes: <br><br>
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 +
==Trypsin==
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Concentration stock solution: 10 mg/200 μL H<sub>2</sub>O<br>
 +
Usual concentration for experiments: μM<br>
 +
Stability: 2-4 weeks <br>
 +
Storage: -20°C <br>
 +
Suppliers: <br>
 +
Incompatibilities: <br>
 +
Notes: 200 μL aliquots<br><br>
 +
 +
==Template==
 +
 +
Molecular weight: g/mol<br>
 +
Concentration stock solution: mM, 1 mg per 1.8 mL  <br>
 +
Usual concentration for experiments: μM<br>
 +
Stability: 2-4 weeks <br>
 +
Storage: -20°C <br>
 +
Suppliers: <br>
 +
Incompatibilities: <br>
Notes: <br><br>
Notes: <br><br>

Revision as of 07:52, 9 December 2009

Contents

Bradykinin

Molecular weight: 1060.21 g/mol
Concentration stock solution: 0.5 mM, 1 mg per 1.8 mL H2O
Usual concentration for experiments: 0.1 μM
Stability: 3-6 months
Storage: -20°C
Suppliers:
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate

Cytosine arabinoside

Molecular weight: 279.68 g/mol
Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: C6645

Notes: 300 μl aliquots

DNAse

Concentration stock solution: 1 mg per 50 μL H2O
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 50 μl aliquots


Glutamine

Molecular weight: 146.14 g/mol
Concentration stock solution: 0.2 M, 29.228 g per 1000 mL
Usual concentration for experiments:
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: 1 ml aliquots

Insulin

Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl
Usual concentration for experiments: μM
Stability: ?
Storage: -20°C
Suppliers: Sigma, I5500
Incompatibilities:
Notes: 200 μl aliqouts


Lanthanum chloride

Molecular weight: 371.37 g/mol (*7H2O)
Concentration stock solution: 0.5M, 186 mg per 1000 mL H2O
Usual concentration for experiments: mM to μM
Stability: >3 months
Storage: +4°C
Suppliers:
Incompatibilities: precipitates with carbonates (buffer!), EGTA and BAPTA have higher affinity to La3+ than to Ca2+
Notes:


Nifedepine

Molecular weight: 346.335 g/mol
Concentration stock solution: 25 mM, 8.66 mg per 1000 μL DMSO
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Notes: N7634 Sigma, very light sensitive, protect solutions from excessive light, preferable fresh stock each day

SKF-96365

Molecular weight:
Concentration stock solution: 50 mM in H2O, 20.15 mg per 1000 μL
Usual concentration for experiments: 1-50 μM
Stability: at least 1 month
Storage: -20°C
Suppliers:
Notes:

Trypsin

Concentration stock solution: 10 mg/200 μL H2O
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 200 μL aliquots

Template

Molecular weight: g/mol
Concentration stock solution: mM, 1 mg per 1.8 mL
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes:

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