User:Maximilian Peters/Stock solutions
From OpenWetWare
| Line 1: | Line 1: | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
==Bradykinin== | ==Bradykinin== | ||
| Line 19: | Line 8: | ||
Suppliers: <br> | Suppliers: <br> | ||
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate<br><br> | Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate<br><br> | ||
| + | |||
| + | ==Cytosine arabinoside== | ||
| + | Molecular weight: 279.68 g/mol<br> | ||
| + | Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml <br> | ||
| + | Stability: ? <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: Sigma<br> | ||
| + | Incompatibilities: <br> | ||
| + | Notes: C6645<br><br> | ||
| + | Notes: 300 μl aliquots<br><br> | ||
| + | |||
| + | ==DNAse== | ||
| + | |||
| + | Concentration stock solution: 1 mg per 50 μL H<sub>2</sub>O<br> | ||
| + | Stability: 2-4 weeks <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: <br> | ||
| + | Incompatibilities: <br> | ||
| + | Notes: 50 μl aliquots <br><br> | ||
| + | |||
| + | |||
| + | ==Glutamine== | ||
| + | |||
| + | Molecular weight: 146.14 g/mol<br> | ||
| + | Concentration stock solution: 0.2 M, 29.228 g per 1000 mL <br> | ||
| + | Usual concentration for experiments: <br> | ||
| + | Stability: ? <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: Sigma<br> | ||
| + | Incompatibilities: <br> | ||
| + | Notes: 1 ml aliquots<br><br> | ||
| + | |||
| + | ==Insulin== | ||
| + | |||
| + | Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl <br> | ||
| + | Usual concentration for experiments: μM<br> | ||
| + | Stability: ? <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: Sigma, I5500<br> | ||
| + | Incompatibilities: <br> | ||
| + | Notes: 200 μl aliqouts <br><br> | ||
| + | |||
==Lanthanum chloride== | ==Lanthanum chloride== | ||
| Line 50: | Line 81: | ||
Storage: -20°C <br> | Storage: -20°C <br> | ||
Suppliers: <br> | Suppliers: <br> | ||
| + | Notes: <br><br> | ||
| + | |||
| + | ==Trypsin== | ||
| + | |||
| + | Concentration stock solution: 10 mg/200 μL H<sub>2</sub>O<br> | ||
| + | Usual concentration for experiments: μM<br> | ||
| + | Stability: 2-4 weeks <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: <br> | ||
| + | Incompatibilities: <br> | ||
| + | Notes: 200 μL aliquots<br><br> | ||
| + | |||
| + | ==Template== | ||
| + | |||
| + | Molecular weight: g/mol<br> | ||
| + | Concentration stock solution: mM, 1 mg per 1.8 mL <br> | ||
| + | Usual concentration for experiments: μM<br> | ||
| + | Stability: 2-4 weeks <br> | ||
| + | Storage: -20°C <br> | ||
| + | Suppliers: <br> | ||
| + | Incompatibilities: <br> | ||
Notes: <br><br> | Notes: <br><br> | ||
Revision as of 07:52, 9 December 2009
Contents |
Bradykinin
Molecular weight: 1060.21 g/mol
Concentration stock solution: 0.5 mM, 1 mg per 1.8 mL H2O
Usual concentration for experiments: 0.1 μM
Stability: 3-6 months
Storage: -20°C
Suppliers:
Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate
Cytosine arabinoside
Molecular weight: 279.68 g/mol
Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: C6645
Notes: 300 μl aliquots
DNAse
Concentration stock solution: 1 mg per 50 μL H2O
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 50 μl aliquots
Glutamine
Molecular weight: 146.14 g/mol
Concentration stock solution: 0.2 M, 29.228 g per 1000 mL
Usual concentration for experiments:
Stability: ?
Storage: -20°C
Suppliers: Sigma
Incompatibilities:
Notes: 1 ml aliquots
Insulin
Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl
Usual concentration for experiments: μM
Stability: ?
Storage: -20°C
Suppliers: Sigma, I5500
Incompatibilities:
Notes: 200 μl aliqouts
Lanthanum chloride
Molecular weight: 371.37 g/mol (*7H2O)
Concentration stock solution: 0.5M, 186 mg per 1000 mL H2O
Usual concentration for experiments: mM to μM
Stability: >3 months
Storage: +4°C
Suppliers:
Incompatibilities: precipitates with carbonates (buffer!), EGTA and BAPTA have higher affinity to La3+ than to Ca2+
Notes:
Nifedepine
Molecular weight: 346.335 g/mol
Concentration stock solution: 25 mM, 8.66 mg per 1000 μL DMSO
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Notes: N7634 Sigma, very light sensitive, protect solutions from excessive light, preferable fresh stock each day
SKF-96365
Molecular weight:
Concentration stock solution: 50 mM in H2O, 20.15 mg per 1000 μL
Usual concentration for experiments: 1-50 μM
Stability: at least 1 month
Storage: -20°C
Suppliers:
Notes:
Trypsin
Concentration stock solution: 10 mg/200 μL H2O
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes: 200 μL aliquots
Template
Molecular weight: g/mol
Concentration stock solution: mM, 1 mg per 1.8 mL
Usual concentration for experiments: μM
Stability: 2-4 weeks
Storage: -20°C
Suppliers:
Incompatibilities:
Notes:
