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| ==[[Cellular Adhesion]]== | | ==[[Cellular Adhesion]]== |
| * PCR | | * No PCR Supermix = no work |
| ** Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4)
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| *** 6His Tag: [[User:Mbennie/Notebook/Oligos/6His_Tag-F|6His_Tag-F]] and [[User:Mbennie/Notebook/Oligos/6His_Tag-R|6His_Tag-R]]
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| *** FLAG Tag: [[User:Mbennie/Notebook/Oligos/FLAG_Tag-F|FLAG_Tag-F]] and [[User:Mbennie/Notebook/Oligos/FLAG_Tag-R|FLAG_Tag-R]]
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| *** Myc Tag: [[User:Mbennie/Notebook/Oligos/Myc_Tag-F|Myc_Tag-F]] and [[User:Mbennie/Notebook/Oligos/Myc_Tag-R|Myc_Tag-R]]
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| *** HA Tag: [[User:Mbennie/Notebook/Oligos/HA_Tag-F|HA_Tag-F]] and [[User:Mbennie/Notebook/Oligos/HA_Tag-R|HA_Tag-R]]
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| *** GCN4 Leucine Zipper: [[User:Mbennie/Notebook/Oligos/GCN4-F|GCN4-F]] and [[User:Mbennie/Notebook/Oligos/GCN4-R2|GCN4-R2]]
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| ** Same protocol as 1.22.2008
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