User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23: Difference between revisions
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** Ran 1% gel for 25 minutes at 95V with samples (5ul sample with 2ul of loading dye) | ** Ran 1% gel for 25 minutes at 95V with samples (5ul sample with 2ul of loading dye) | ||
** Not a great gel, but the smudges around 100 bp look promising | ** Not a great gel, but the smudges around 100 bp look promising | ||
** '''Extra band in GCN4 sample''' | |||
[[Image:Mbennie2008.1.23.jpg|thumb|log2-ladder, 6His, FLAG, myc, HA, GCN4]] | [[Image:Mbennie2008.1.23.jpg|thumb|log2-ladder, 6His, FLAG, myc, HA, GCN4]] | ||
Revision as of 10:05, 23 January 2008
Cellular Adhesion
- Gel
- Ran 1% gel for 25 minutes at 95V with samples (5ul sample with 2ul of loading dye)
- Not a great gel, but the smudges around 100 bp look promising
- Extra band in GCN4 sample
- PCR Purification
- Used MinElute columns to purify the five tags and one zipper from 1.22 PCR
- Eluted in 10ul of water
- Digest
- Ran on PCR purification above
- Template for tags: 3ul DNA (each), 2ul NEB2, .5ul EcoR1, .5ul Pst1, rest water (20ul rxn)
- Thermocycler protocol: 1hr@37C, 20mins@80C
