User:Md Rezaul Karim/Notebook/Radioligand binding assay

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==Project Description/Abstract==
==Project Description/Abstract==
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* Place short description of project or notes regarding this project
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* Applying different ligand-receptor binding assay techniques on the membrane preparations from rat/mouse brain/adrenal glands/pancreas/kidney and Ang II receptor autoradiography, this lab finds out the subtype of Ang II receptors present there and their binding affinity as well as specificity.
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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* Lab – Dr. Robert C. Speth
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* Duration- 1/07/13 - 2/01/13
==Notes==
==Notes==
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* Place some notes here for visitors
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* Buffer solution: The recipe for the 0.5L assay buffer (Assay Medium or AM) is:
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NaCl 4.38 g
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Na2EDTA 930 mg
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Bacitracin 70.5 mg
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500mM dibasic NaPO4 50 ml
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Distilled deionized water 450 ml
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pH is adjusted to 7.1-7.2 with HCl
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* Hypotonic buffer solution: The recipe for the 0.5L hypotonic buffer solution is:
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500mM NaH2PO4         12 ml
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200mM Na2HPO4         70 ml
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Distilled deionized water 918 ml
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pH was checked to be 7.15
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* Example: This project is currently on hold until further notice.
 

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Project Description/Abstract

  • Applying different ligand-receptor binding assay techniques on the membrane preparations from rat/mouse brain/adrenal glands/pancreas/kidney and Ang II receptor autoradiography, this lab finds out the subtype of Ang II receptors present there and their binding affinity as well as specificity.
  • Lab – Dr. Robert C. Speth
  • Duration- 1/07/13 - 2/01/13

Notes

  • Buffer solution: The recipe for the 0.5L assay buffer (Assay Medium or AM) is:

NaCl 4.38 g Na2EDTA 930 mg Bacitracin 70.5 mg 500mM dibasic NaPO4 50 ml Distilled deionized water 450 ml pH is adjusted to 7.1-7.2 with HCl

  • Hypotonic buffer solution: The recipe for the 0.5L hypotonic buffer solution is:

500mM NaH2PO4 12 ml 200mM Na2HPO4 70 ml Distilled deionized water 918 ml pH was checked to be 7.15



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