User:Megan E. Garber/Notebook/BioE140L lab notebook/2013/04/04

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(Entry title)
Current revision (12:09, 4 April 2013) (view source)
(April 4th 2013)
 
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==April 4th 2013==
==April 4th 2013==
-
Ligations
+
'''Ligations'''
*6.5ul water
*6.5ul water
*1 ul Ligation Buffer
*1 ul Ligation Buffer
Line 14: Line 14:
*0.5 ul Ligase
*0.5 ul Ligase
-
Transormations
+
'''Transformations'''
*1 tube cells
*1 tube cells
*30 ul KCM
*30 ul KCM

Current revision

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April 4th 2013

Ligations

  • 6.5ul water
  • 1 ul Ligation Buffer
  • 1 ul vector
  • 1ul insert
  • 0.5 ul Ligase

Transformations

  • 1 tube cells
  • 30 ul KCM
  • no water



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