# User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/01/29

(Difference between revisions)
 Revision as of 01:06, 7 February 2014 (view source) (→Entry title)← Previous diff Current revision (01:06, 7 February 2014) (view source) (→Calculations) Line 18: Line 18: *Myoglobin stock solution: *Myoglobin stock solution: **0.011g x (1 mol/17699g) x 0.010L = 0.0621mM **0.011g x (1 mol/17699g) x 0.010L = 0.0621mM - *Volumes of Au, Myoglobin, and water for each test tube: + *Volumes of Au, Myoglobin, and water for each test tube were 5mL - **Final [Au]=0.25mM, 5mL total volume + [[Image:2014_0129_Au_Myo_NPs_cheat_sheet.PNG|800px]] [[Image:2014_0129_Au_Myo_NPs_cheat_sheet.PNG|800px]]

## Current revision

Project name Main project page
Previous entry      Next entry

## Method

• There were a total of 12 gold: nanofiber solutions made, with all of them being the same
• The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM
• Three of the solutions will have the scaffolds in them as this is our first method
• The other 9 will be used to make the other two methods
• They were put in an oven for 4hrs at 80°C

## Calculations

• Au stock solution:
• 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
• Myoglobin stock solution:
• 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
• Volumes of Au, Myoglobin, and water for each test tube were 5mL