User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/01/29

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(Entry title)
Current revision (00:06, 7 February 2014) (view source)
(Calculations)
 
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*Myoglobin stock solution:
*Myoglobin stock solution:
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
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*Volumes of Au, Myoglobin, and water for each test tube:
+
*Volumes of Au, Myoglobin, and water for each test tube were 5mL
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**Final [Au]=0.25mM, 5mL total volume
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[[Image:2014_0129_Au_Myo_NPs_cheat_sheet.PNG|800px]]<br.>
[[Image:2014_0129_Au_Myo_NPs_cheat_sheet.PNG|800px]]<br.>

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Method

  • There were a total of 12 gold: nanofiber solutions made, with all of them being the same
  • The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM
  • Three of the solutions will have the scaffolds in them as this is our first method
  • The other 9 will be used to make the other two methods
  • They were put in an oven for 4hrs at 80°C

Calculations

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, Myoglobin, and water for each test tube were 5mL




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