# User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/04

(Difference between revisions)
 Revision as of 01:13, 7 February 2014 (view source) (→Results From Last Time)← Previous diff Current revision (01:16, 7 February 2014) (view source) (→Calculations) Line 26: Line 26: *Volumes of Au, Myoglobin, and water for each test tube: *Volumes of Au, Myoglobin, and water for each test tube: **Final [Au]=0.5mM, 5mL total volume **Final [Au]=0.5mM, 5mL total volume - [ + *For the new solutions, one will have a tall scaffold, another with four scaffold that equal the tall scaffold, and the third one will have a short one - + *The other nine solutions will be used for later testing + *The 12 were put in the oven for 4hrs at 80°C |} |} __NOTOC__ __NOTOC__

## Current revision

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## Entry title

• Nanofibers formed but were not the color that they were suppose to be
• The concentration of gold and myoglobin were increased and new solutions were made
• Taller scaffolds were printed, so the entire solution had scaffold present

## Results From Last Time

• 100:1 Gold:Myoglobin Nanofibers alone
• 100:1 Gold:Myoglobin Nanofibers on PLA scaffolds

• 100:1 Gold:Myoglobin Nanofbiers on PLA sacffold- under microscope

## Calculations

• For the new Au:Myoglobin solutions, we used:

• Au stock solution:
• 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
• Myoglobin stock solution:
• 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
• Volumes of Au, Myoglobin, and water for each test tube:
• Final [Au]=0.5mM, 5mL total volume
• For the new solutions, one will have a tall scaffold, another with four scaffold that equal the tall scaffold, and the third one will have a short one
• The other nine solutions will be used for later testing
• The 12 were put in the oven for 4hrs at 80°C