User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/12

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(Autocreate 2014/02/12 Entry for User:Megan_L._Channell/Notebook/3D_printing_scaffolds_with_Au_nanofibers)
Current revision (16:42, 20 February 2014) (view source)
 
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==Entry title==
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===Objectives===
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* Insert content here...
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*Pictures of nanofibers from yesterday: macro and micro
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*Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
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*Brute force method with 0.5mM gold-Myoglobin nanofibers
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*Autoclave one dry, one wet scaffold
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===Myoglobin and BSA Nanofiber Results===
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*160:1 BSA
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[[Image:Photo_Feb_12,_12_17_16_PM.jpg|300px]]
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*120:1 BSA
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[[Image:Photo_Feb_12,_12_17_31_PM.jpg|300px]]
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*80:1 BSA
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[[Image:Photo_Feb_12,_12_17_41_PM.jpg|300px]]
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*100:1 Myoglobin
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[[Image:Photo_Feb_12,_12_17_50_PM.jpg|300px]]
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*60:1 Myoglobin
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[[Image:Photo_Feb_12,_12_18_06_PM.jpg|300px]]
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===Nanofiber Retention Tests===
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*For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
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#Sonicate for 30 minutes
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#Heat at 30C for 30 minutes
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#Ethanol
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#PBS
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#strong acid
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====Nanofiber Retention Test Results (February 19, 2014)====
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(tests pictured, from left to right: strong acid, ethanol, PBS, sonicated)<br.>
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*160:1 BSA
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[[Image:Photo_Feb_19,_2_59_58_PM.jpg|300px]]<br.>
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<br.>
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[[Image:Photo_Feb_19,_3_00_06_PM.jpg|400px]]
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*120:1 BSA
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[[Image:Photo_Feb_19%2C_2_57_38_PM.jpg|300px]]<br.>
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<br.>
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[[Image:Photo_Feb_19,_2_57_50_PM.jpg|400px]]
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*80:1 BSA
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[[Image:Photo_Feb_19,_2_58_48_PM.jpg|300px]]<br.>
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<br.>
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[[Image:Photo_Feb_19,_2_58_54_PM.jpg|400px]]
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*60:1 Myoglobin
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[[Image:Photo_Feb_19,_2_56_39_PM.jpg|300px]]<br.>
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<br.>
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[[Image:Photo_Feb_19,_2_56_50_PM.jpg|400px]]
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===Autoclaved Scaffolds===
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*PLA scaffolds were dry autoclaved
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*Scaffolds did not melt as expected
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[[Image:Photo_Feb_12,_2_49_20_PM.jpg|300px]]
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Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

  • 120:1 BSA

  • 80:1 BSA

  • 100:1 Myoglobin

  • 60:1 Myoglobin

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid

Nanofiber Retention Test Results (February 19, 2014)

(tests pictured, from left to right: strong acid, ethanol, PBS, sonicated)

  • 160:1 BSA



  • 120:1 BSA



  • 80:1 BSA



  • 60:1 Myoglobin



Autoclaved Scaffolds

  • PLA scaffolds were dry autoclaved
  • Scaffolds did not melt as expected


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