User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18

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==Entry title==
==Entry title==
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* Insert content here...
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* Make polydopamine for nanofibers
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*Myoglobin for procedure
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==Procedure==
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*Preparing scaffolds
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**Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
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***First two scaffolds immersed in solution pH 8.51
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***Second two scaffolds immersed in solution pH 8.59
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**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
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**Rinse with deionized water and allow scaffold to dry overnight
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==Results==
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*Brute force
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[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]
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[[Image:Bforce_myo.jpg|300px]]<br.>
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[[Image:Bforce_myo_10x3.jpg|300px]] 
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[[Image:Bforce_myo3.jpg|300px]]<br.>
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*Scaffolds after 1 hr in Tris-polydopamine
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[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]
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*Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution

Revision as of 15:47, 20 February 2014

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Entry title

  • Make polydopamine for nanofibers
  • Myoglobin for procedure

Procedure

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Results

  • Brute force



  • Scaffolds after 1 hr in Tris-polydopamine

  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution



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