User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18: Difference between revisions
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(Autocreate 2014/02/18 Entry for User:Megan_L._Channell/Notebook/3D_printing_scaffolds_with_Au_nanofibers) |
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==Entry title== | ==Entry title== | ||
* | * Make polydopamine for nanofibers | ||
*Myoglobin for procedure | |||
==Procedure== | |||
*Preparing scaffolds | |||
**Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour | |||
***First two scaffolds immersed in solution pH 8.51 | |||
***Second two scaffolds immersed in solution pH 8.59 | |||
**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours | |||
**Rinse with deionized water and allow scaffold to dry overnight | |||
==Results== | |||
*Brute force | |||
[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]] | |||
[[Image:Bforce_myo.jpg|300px]]<br.> | |||
[[Image:Bforce_myo_10x3.jpg|300px]] | |||
[[Image:Bforce_myo3.jpg|300px]]<br.> | |||
*Scaffolds after 1 hr in Tris-polydopamine | |||
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]] | |||
*Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution | |||
Revision as of 13:47, 20 February 2014
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