User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18

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*Brute force
*Brute force
[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]  
[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]  
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[[Image:Bforce_myo.jpg|300px]]<br.>
+
[[Image:Bforce_myo.jpg|300px]]
[[Image:Bforce_myo_10x3.jpg|300px]]   
[[Image:Bforce_myo_10x3.jpg|300px]]   
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[[Image:Bforce_myo3.jpg|300px]]<br.>
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[[Image:Bforce_myo3.jpg|300px]]
*Scaffolds after 1 hr in Tris-polydopamine
*Scaffolds after 1 hr in Tris-polydopamine
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]

Revision as of 15:47, 20 February 2014

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Entry title

  • Make polydopamine for nanofibers
  • Myoglobin for procedure

Procedure

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Results

  • Brute force

  • Scaffolds after 1 hr in Tris-polydopamine

  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution



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