User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/19: Difference between revisions
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== | ===Objectives=== | ||
* | *Finish polydopamine nanofiber scaffolds | ||
*Wet-autoclave nanofiber coated scaffold in PBS | |||
*Attempt to make silver:protein nanofibers | |||
===Sample Preparation: Polydopamine nanofibers=== | |||
*Au stock solution: | |||
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM | |||
*Myoglobin stock solution: | |||
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM | |||
*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11] pictured below): | |||
**Final [Au]=0.5mM, 5mL total volume | |||
**[[Image:2014_0211_myo_bsa_NPs.PNG]] | |||
===Autoclaved Nanofiber-coated Scaffold=== | |||
*Nanofiber coated scaffold from February 5 wet-autoclaved in PBS | |||
**Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained | |||
**This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS | |||
Macro image:<br.> | |||
[[Image:Photo_Feb_19,_2_11_50_PM.jpg|300px]]<br.> | |||
Micro images:<br.> | |||
[[Image:Test1PBS_autoclav_myo10x.jpg|400px]]<br.> | |||
[[Image:Test1PBS_autoclav_myo10x3.jpg|400px]]<br.> | |||
===Sample Preparation: Silver:BSA Nanofiber=== | |||
*We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work) | |||
*The final solution will have 0.25mM AgNO<sub>3</sub>, 1mM HCl, water, and defined ratios of BSA as detailed in the table below | |||
*All samples will be heated to 80C in an oven for 4 hours | |||
[[Image:2014_0219_Ag_BSA_nanofibers_ratios.PNG|900px]] | |||
Revision as of 13:49, 20 February 2014
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Objectives
Sample Preparation: Polydopamine nanofibers
Autoclaved Nanofiber-coated Scaffold
Macro image:<br.> <br.> Micro images:<br.> <br.> <br.> Sample Preparation: Silver:BSA Nanofiber
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