User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/19

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(Autocreate 2014/02/19 Entry for User:Megan_L._Channell/Notebook/3D_printing_scaffolds_with_Au_nanofibers)
Current revision (15:49, 20 February 2014) (view source)
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==Entry title==
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===Objectives===
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* Insert content here...
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*Finish polydopamine nanofiber scaffolds
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*Wet-autoclave nanofiber coated scaffold in PBS
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*Attempt to make silver:protein nanofibers
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===Sample Preparation: Polydopamine nanofibers===
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*Au stock solution:
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**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
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*Myoglobin stock solution:
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**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
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*Volumes of Au, protein, and water for each test tube (Myoglobin ratios from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/11#New_Nanofibers:_Sample_Preparation Febrary 11] pictured below):
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**Final [Au]=0.5mM, 5mL total volume
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**[[Image:2014_0211_myo_bsa_NPs.PNG]]
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===Autoclaved Nanofiber-coated Scaffold===
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*Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
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**Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
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**This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS
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Macro image:<br.>
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[[Image:Photo_Feb_19,_2_11_50_PM.jpg|300px]]<br.>
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Micro images:<br.>
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[[Image:Test1PBS_autoclav_myo10x.jpg|400px]]<br.>
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[[Image:Test1PBS_autoclav_myo10x3.jpg|400px]]<br.>
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===Sample Preparation: Silver:BSA Nanofiber===
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*We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
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*The final solution will have 0.25mM AgNO<sub>3</sub>, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
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*All samples will be heated to 80C in an oven for 4 hours
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[[Image:2014_0219_Ag_BSA_nanofibers_ratios.PNG|900px]]
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Objectives

  • Finish polydopamine nanofiber scaffolds
  • Wet-autoclave nanofiber coated scaffold in PBS
  • Attempt to make silver:protein nanofibers

Sample Preparation: Polydopamine nanofibers

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11 pictured below):
    • Final [Au]=0.5mM, 5mL total volume
    • Image:2014_0211_myo_bsa_NPs.PNG

Autoclaved Nanofiber-coated Scaffold

  • Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
    • Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
    • This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS

Macro image:

Micro images:


Sample Preparation: Silver:BSA Nanofiber

  • We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
  • The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
  • All samples will be heated to 80C in an oven for 4 hours



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