User:Megan L. Channell/Notebook/Horseradish/2013/09/11: Difference between revisions

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==Protocol==
==Protocol==
The protocol can be found from [[User:Megan L. Channell/Notebook/Horseradish/2013/09/10\yesterdays]] lab.  The only adjestment was that the diltuion of the HRP was a total of 10 mL instead of 2mL.
The protocol can be found from [[User:Megan L. Channell/Notebook/Horseradish/2013/09/10|yesterdays]] lab.  The only adjestment was that the diltuion of the HRP was a total of 10 mL instead of 2mL.


==Data==
==Data==

Revision as of 19:38, 16 September 2013

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Horseradish Peroxidase UV-Vis and Gold Solution

Objective

Determine Horseradish Peroxidase (HRP) concentration through a calibration curve. This is done through the Bradford Assay.

Protocol

The protocol can be found from yesterdays lab. The only adjestment was that the diltuion of the HRP was a total of 10 mL instead of 2mL.

Data

  • UV-VIs HRP for Trial 1 and 2

  • Calibration curve of HRP for Trial 1 and 2

Note

Trial 1 and 2 were done with a cuvette that had a path length of .412 cm. Trial 1 and 2 were not accurate when looking at the calibration curve. A new set of standards will be made for tomorrow.



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