User:Megan L. Channell/Notebook/Horseradish/2013/09/18: Difference between revisions
From OpenWetWare
(Autocreate 2013/09/18 Entry for User:Megan_L._Channell/Notebook/Horseradish) |
(fix raw html notebook nav) |
||
(7 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->==Oxidation and Reduction HRP== | ||
== | |||
* | ==Objective== | ||
Perform a redox titration on Horseradish Peroxidase to determine the standard potential. | |||
==Protocol== | |||
The procedure used for the lab comes from Dr. Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/18 | lab notebook]]. A total of 5 groups analyzed the cell potential. Group CMJ went first and in the middle of the five runs, the amount of sodium dithionite added to the cell was adjusted. The amounts and concentrations of sodium dithionite added were kept for each group. | |||
==Data== | |||
The class data follows below: | |||
[[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_table.png]] | |||
[[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_graph.png]] | |||
==Calculations== | |||
*To determine % oxidized, the absorbance peak of 405.32 nm was used from the class data. | |||
*[(Absorbance Value for data pt- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100=% oxidized | |||
* The % Oxidized was subtracted from 100 to calculate the % reduced. | |||
Latest revision as of 23:23, 26 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
Oxidation and Reduction HRPObjectivePerform a redox titration on Horseradish Peroxidase to determine the standard potential. ProtocolThe procedure used for the lab comes from Dr. Hartings lab notebook. A total of 5 groups analyzed the cell potential. Group CMJ went first and in the middle of the five runs, the amount of sodium dithionite added to the cell was adjusted. The amounts and concentrations of sodium dithionite added were kept for each group. DataCalculations
|