User:Megan L. Channell/Notebook/Horseradish/2013/09/24: Difference between revisions

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==Entry title==
 
* Insert content here...
 
 
 
==Objective==
Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin.  The data collected will be used in a future experiment.
 
==Protocol==
The procedure for the lab can be found in Dr. Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]].
*Noted differences:
**Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid. 
**For the concnetration of pepstatin added, the group decided upon 0.2 μM.
**The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
 
==Data==
* UV-VIS spectra data, taken at 1/2 hour intervals
[[Image:9.24.13 cmj UVVIS pepsinpepstatin.png|800 px|]]
 




Latest revision as of 23:23, 26 September 2017

Biomaterials Design Lab Main project page
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Objective

Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment.

Protocol

The procedure for the lab can be found in Dr. Hartings lab notebook.

  • Noted differences:
    • Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.
    • For the concnetration of pepstatin added, the group decided upon 0.2 μM.
    • The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
    • For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.

Data

  • UV-VIS spectra data, taken at 1/2 hour intervals



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