User:Megan L. Channell/Notebook/Horseradish/2013/09/24

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Objective

Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment.

Protocol

The procedure for the lab can be found in Dr. Harting's lab notebook. One of noted difference is instead of 1.7 M perchloric acid, it was 1 M of perchloric acid. For the concnetration of pepstatin added, the group decided upon 0.2 μM. The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin) For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.



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