User:Megan L. Channell/Notebook/Horseradish/2013/09/25

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Data)
(Protocol)
Line 15: Line 15:
==Protocol==
==Protocol==
-
The procedure came from Dr. Hartings' [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 | la notebook]].
+
The procedure came from Dr. Hartings' [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 | la notebook]].  Two additional samples were made using the 2 nM of pepsin and  0.2μM of pepstatin solutions yesterday.  It was prepared just like the SDS-PAGE samples except stored in a refrigerator.
==Data==
==Data==

Revision as of 20:38, 30 September 2013

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

Run SDS-PAGE on the amount of hemoglobin from yesterday's lab.

Protocol

The procedure came from Dr. Hartings' la notebook. Two additional samples were made using the 2 nM of pepsin and 0.2μM of pepstatin solutions yesterday. It was prepared just like the SDS-PAGE samples except stored in a refrigerator.

Data

Observations of the SDS-PAGE with the samples from yesterday.

Image:9 25 2013 cmj SDSPAGE gel.png

  • For the wells going left to right:
  1. pepstatin 1
  2. pepsin 1
  3. pepsin 2
  4. pepstatin 4
  5. hemoglobin
  6. pepstatin 3
  7. pepsin 3
  8. pepsin 4
  9. pepstatin 2
  10. pepsin stock
  11. pepstatin stock


Personal tools