User:Megan L. Channell/Notebook/Horseradish/2013/10/01

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(Autocreate 2013/10/01 Entry for User:Megan_L._Channell/Notebook/Horseradish)
Current revision (14:28, 10 October 2013) (view source)
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==Entry title==
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
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To monitor the kinetics and yield of the HRP-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles.  
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From [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/01 | Dr. Hartings]]
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==Procedure==
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'''1. UV-Vis Absorbance of Reactants, Catalysts, and Products'''
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# Take UV-Vis of horseradish peroxidase stock solution.
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# Take UV-Vis of luminol of stock solution. The absorbance too high, so the stock solution was diluted 200x.
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# Take UV-Vis of 3-aminophthalic acid.
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:* Data not taken.
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==Figures==
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[[Image:HRP-luminol-H2O2-UVVIS.jpg|650 px|]]
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==Notes==
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Objective

To monitor the kinetics and yield of the HRP-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles.

From Dr. Hartings


Procedure

1. UV-Vis Absorbance of Reactants, Catalysts, and Products

  1. Take UV-Vis of horseradish peroxidase stock solution.
  2. Take UV-Vis of luminol of stock solution. The absorbance too high, so the stock solution was diluted 200x.
  3. Take UV-Vis of 3-aminophthalic acid.
  • Data not taken.

Figures

Notes


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