User:Megan L. Channell/Notebook/Horseradish/2013/10/09: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
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== | |||
* | |||
==Objective== | |||
Continue work from yesterday, but to observe the ADA kinetics with an inhibitor. | |||
==Procedure== | |||
'''Creating the Adenosine Stock''' | |||
# Add 0.1073 g of adenosine to a 10 ml volumetric flask. Fill with 50 mM phosphate buffer at pH 7.4. This created a stock solution of 0.04 M. | |||
# Add 10.00 μL of the 0.04 M adenosine stock to a 10 mL volumetric flask. Fill with 50 mM phosphate buffer at pH 7.4. This created a final solution of 40 μM. | |||
'''Enzyme Kinetics Measurement''' | |||
# Add 3 mL of 40 μM adenosine solution to the cuvette and 30 μL of inosine. | |||
# Start kinetics measurement: | |||
:* 1 ms integration | |||
:* 10 scan average | |||
:* Set "Save the first available scan every" to 15 seconds | |||
:* Set "Stop after this amount of time" to 10 minutes | |||
:* Set "File Type" to Tab Delimited | |||
:* Just before 1 minute, add 1.5 μL of 1 nM EHNA. | |||
==Data== | |||
[[Image:Cmj_10.09.13_ADAwithinhibitor.png]] | |||
==Notes== | |||
This area is for any observations or conclusions that you would like to note. | |||
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. | |||
[[Category:CHEM571]] | |||
[[Category:Adenosine]] | |||
[[Category:Inosine]] | |||
Revision as of 12:35, 10 October 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveContinue work from yesterday, but to observe the ADA kinetics with an inhibitor. ProcedureCreating the Adenosine Stock
Enzyme Kinetics Measurement
DataNotesThis area is for any observations or conclusions that you would like to note.
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