User:Megan L. Channell/Notebook/Horseradish/2013/10/15: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...


==Objective==
To make HRP-AuNPs for enzymatic studies.
==Procedure==
'''Creating the Solutions of Gold and HRP'''
# For 30:1 Au:HRP solution, 5.10 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
# For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
# For 90:1 Au:HRP solution, 1.71 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
# For 120:1 Au:HRP solution, 1.28 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
'''Synthesis of HRP-AuNPs'''
# The 10 mL solutions of gold and HRP were placed into four different test tubes.
# The test tubes were placed in the oven at 80°C for approximately 3 hours.
:* From 12:40pm to 4:00pm.
==Notes==
* The stock solution of gold was 2.94 mM.
* The stock solution of HRP was 16.27 μM.


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Revision as of 16:19, 15 October 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To make HRP-AuNPs for enzymatic studies.

Procedure

Creating the Solutions of Gold and HRP

  1. For 30:1 Au:HRP solution, 5.10 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  2. For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  3. For 90:1 Au:HRP solution, 1.71 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  4. For 120:1 Au:HRP solution, 1.28 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.

Synthesis of HRP-AuNPs

  1. The 10 mL solutions of gold and HRP were placed into four different test tubes.
  2. The test tubes were placed in the oven at 80°C for approximately 3 hours.
  • From 12:40pm to 4:00pm.

Notes

  • The stock solution of gold was 2.94 mM.
  • The stock solution of HRP was 16.27 μM.


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