User:Melissa Novy/Notebook/CHEM-571/2012/09/04

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(Tris Buffer Solutions)
Current revision (22:17, 21 September 2012) (view source)
(Objectives)
 
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==Objectives==
==Objectives==
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* Analyze AuNP+BSA solutions with UV-vis spectroscopy.
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* Analyze AuNP/BSA solutions with UV-vis spectroscopy. Data will be posted next week.
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* Remake AuNP+BSA solutions that did not produce the expected peaks on their UV-vis spectra.
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* Make Tris buffer solutions at pH values of 8 and 10.
* Make Tris buffer solutions at pH values of 8 and 10.
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* Refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04|Dhea Patel's entry]] for UV-vis data.
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* Refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04|Dhea Patel's entry]] for AuNP/BSA solution ratios, calculations, and images.
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[[User:Abigail E. Miller|Abigail E. Miller]] 09:45, 17 September 2012 (EDT) there is no indication in your notebook that you completed the second objective. what data exactly is available in Dhea's notebook? Absorbance measurements of what and why?
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==Tris Buffer Solutions==
==Tris Buffer Solutions==

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Objectives

  • Analyze AuNP/BSA solutions with UV-vis spectroscopy. Data will be posted next week.
  • Make Tris buffer solutions at pH values of 8 and 10.
  • Refer to Dhea Patel's entry for AuNP/BSA solution ratios, calculations, and images.

Tris Buffer Solutions

  • 100 mL of 0.1 M Tris buffer solution requires:
  MW Tris: 121.14 g/mol
  (0.1 mol Tris/1 L) × (121.14 g Tris/1 mol Tris) = 12.114 g Tris/L
  (12.114 g Tris/1 L) × 0.1 L = 1.2114 g Tris/0.1 L
  Theoretical mass of Tris: 1.2114 g
  • 1.2114 g of Tris buffer was added to 100 mL of H2O to make a 0.1 M Tris buffer solution.
  • While stirring to ensure even distribution of particles, the pH of the solution was adjusted to 8.0 with drops of 1 M HCl. The total volume of HCl used was about 7 mL.
  • Another 100 mL of 0.1 M Tris buffer solution was made and its pH was adjusted to 10.0 with drops of 1 M HCl. The total volume of HCl used was about 1 mL.

Absorbance Data

Image:9-4-12_Absorbance_AuNP-BSA.JPG

  • Absorbance of AuNP/BSA solutions at concentrations ranging from 60 μM to 170 μM to determine the formation of AuNPs.
  • A peak at around 530 nm for the 80 μM solution indicates the presence of AuNPs in solution. A visual confirmation was that the solution was also transparent purple in color.
  • No peaks occurred for the other solutions, because no AuNPs formed. These solutions contained yellow-brown precipitate instead. The gold likely did not react with the BSA in the manner intended.

Notes

  • The original AuNP solutions did not form nanoparticles, as the HAuCl4 reacted with the metal spatula. They were yellow in color and the higher ratio solutions contained brown precipitate.
  • Visual analysis indicated that all but the 60 and 80 Au/BSA solutions of the second set of AuNP+BSA solutions contained fibers.
  • Please refer to Keyun Wang's entry for more observations about the solutions.



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