User:Melissa Novy/Notebook/CHEM-571/2012/09/05 - Revision history

Melissa Novy: /* UV-Vis Analysis */

2012-10-03T02:35:15Z

UV-Vis Analysis

←Older revision		Revision as of 02:35, 3 October 2012
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	* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.		* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.
	* The presence of a peak at around 540 nm for all solutions indicates that at least some AuNPs became resuspended in solution. However, as no absorbance spectra of pure Tris buffer has been taken, it may be that the peaks are a result of absorbance by the buffer.		* The presence of a peak at around 540 nm for all solutions indicates that at least some AuNPs became resuspended in solution. However, as no absorbance spectra of pure Tris buffer has been taken, it may be that the peaks are a result of absorbance by the buffer.
-		+	** Please refer to the entry on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/26\|2012/09/26]] for UV-vis spectra of Tris buffers and data analysis.
-	*~~'''~~[[User:~~Abigail E. Miller~~\|~~Abigail E. Miller~~]] ~~09:51, 17 September 2012 (EDT)''':why didn't you measure~~ Tris buffer? plot absorbance versus tris buffer concentration? does that show any trend? you need to repeat this and demonstrate whether it is due to tris or not. First measure Tris buffer absorbance spectrum to see if it even abosrbance around 530 nm. then Measure Tris buffer as blanks (at each concentration) and then measure solutions with AuNPs right after mixing and ~~then later~~.	+

	==Notes==		==Notes==

Melissa Novy: /* Au/BSA Fibers */

2012-09-22T02:24:45Z

Au/BSA Fibers

←Older revision		Revision as of 02:24, 22 September 2012
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	==Au/BSA Fibers==		==Au/BSA Fibers==
	* Protocol for Resuspending Au/BSA Fibers		* Protocol for Resuspending Au/BSA Fibers
-	*# Au/BSA solutions were centrifuged at 3000 rpm, 25°C, for 5 min, such that the fibers formed a pellet at the bottom of the centrifuge tube.	+	*# Au/BSA solutions made on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31\|2012/08/31 by Dr. Miller]] were obtained.
		+	*# The solutions were centrifuged at 3000 rpm, 25°C, for 5 min, such that the fibers formed a pellet at the bottom of the centrifuge tube.
	*# 1 mL of 10 mM pH 8 Tris buffer was added to each pellet and pipetted up and down to resuspend the fibers.		*# 1 mL of 10 mM pH 8 Tris buffer was added to each pellet and pipetted up and down to resuspend the fibers.
	*# Step two was repeated with Tris buffer at concentrations of 1 mM, 100 μM, and 10 μM on fibers with arbitrary Au/BSA mole ratios.		*# Step two was repeated with Tris buffer at concentrations of 1 mM, 100 μM, and 10 μM on fibers with arbitrary Au/BSA mole ratios.
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	* UV-vis spectroscopy was conducted on the resuspended fiber solutions.		* UV-vis spectroscopy was conducted on the resuspended fiber solutions.
-		+	* The AuNP/BSA solution ratios were considered arbitrary for this analysis, as the objective was to determine only whether AuNP/BSA fibers could be resuspended in Tris buffer. The presence of a peak at around 540 nm indicated the presence of AuNPs and therefore successful resuspension of the fibers.
-	~~[[User:Abigail E~~. ~~Miller\|Abigail E~~. ~~Miller]] 09:46, 17 September 2012 (EDT) what fiber solutions did you use? from what ratios?~~	+

	==UV-Vis Analysis==		==UV-Vis Analysis==

Abigail E. Miller: /* UV-Vis Analysis */

2012-09-17T13:51:48Z

UV-Vis Analysis

←Older revision		Revision as of 13:51, 17 September 2012
Line 35:		Line 35:
	* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.		* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.
	* The presence of a peak at around 540 nm for all solutions indicates that at least some AuNPs became resuspended in solution. However, as no absorbance spectra of pure Tris buffer has been taken, it may be that the peaks are a result of absorbance by the buffer.		* The presence of a peak at around 540 nm for all solutions indicates that at least some AuNPs became resuspended in solution. However, as no absorbance spectra of pure Tris buffer has been taken, it may be that the peaks are a result of absorbance by the buffer.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 09:51, 17 September 2012 (EDT)''':why didn't you measure Tris buffer? plot absorbance versus tris buffer concentration? does that show any trend? you need to repeat this and demonstrate whether it is due to tris or not. First measure Tris buffer absorbance spectrum to see if it even abosrbance around 530 nm. then Measure Tris buffer as blanks (at each concentration) and then measure solutions with AuNPs right after mixing and then later.

	==Notes==		==Notes==

Abigail E. Miller: /* Au/BSA Fibers */

2012-09-17T13:46:36Z

Au/BSA Fibers

←Older revision		Revision as of 13:46, 17 September 2012
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	* UV-vis spectroscopy was conducted on the resuspended fiber solutions.		* UV-vis spectroscopy was conducted on the resuspended fiber solutions.
		+
		+	[[User:Abigail E. Miller\|Abigail E. Miller]] 09:46, 17 September 2012 (EDT) what fiber solutions did you use? from what ratios?

	==UV-Vis Analysis==		==UV-Vis Analysis==

Melissa Novy: /* UV-Vis Analysis */

2012-09-14T03:24:00Z

UV-Vis Analysis

←Older revision		Revision as of 03:24, 14 September 2012
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	[[Image:9-5-12_Absorbance_AuNP-BSA_Tris_Buffer.JPG]]		[[Image:9-5-12_Absorbance_AuNP-BSA_Tris_Buffer.JPG]]
	* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.		* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.
		+	* The presence of a peak at around 540 nm for all solutions indicates that at least some AuNPs became resuspended in solution. However, as no absorbance spectra of pure Tris buffer has been taken, it may be that the peaks are a result of absorbance by the buffer.

	==Notes==		==Notes==

Melissa Novy: /* Notes */

2012-09-11T18:12:54Z

Notes

←Older revision		Revision as of 18:12, 11 September 2012
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	==Notes==		==Notes==
	* Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/05\|Keyun Wang's entry]] for observations on making and centrifuging Au/BSA solutions.		* Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/05\|Keyun Wang's entry]] for observations on making and centrifuging Au/BSA solutions.
-		+	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/05\|Dhea Patel's entry]] for volumes and calculations used in remaking the Au/BSA solutions.
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	__NOTOC__		__NOTOC__

Melissa Novy: /* UV-Vis Analysis */

2012-09-11T17:58:55Z

UV-Vis Analysis

←Older revision		Revision as of 17:58, 11 September 2012
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	==UV-Vis Analysis==		==UV-Vis Analysis==
-	* ~~Graph~~	+	[[Image:9-5-12_Absorbance_AuNP-BSA_Tris_Buffer.JPG]]
		+	* Absorbance of AuNP/BSA fibers resuspended in Tris buffer at concentrations of 100 μM, 10 μM, 1 mM, and 10 mM and pH of 8 or 10.

	==Notes==		==Notes==

Melissa Novy: /* Notes */

2012-09-05T19:28:24Z

Notes

←Older revision		Revision as of 19:28, 5 September 2012
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	==Notes==		==Notes==
-	* ~~Refer~~ to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/05\|Keyun Wang's entry]] for observations on making and centrifuging Au/BSA solutions.	+	* Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/05\|Keyun Wang's entry]] for observations on making and centrifuging Au/BSA solutions.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Melissa Novy: /* Au/BSA Fibers */

2012-09-05T19:27:42Z

Au/BSA Fibers

←Older revision		Revision as of 19:27, 5 September 2012
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	*# Step two was repeated with Tris buffer at concentrations of 1 mM, 100 μM, and 10 μM on fibers with arbitrary Au/BSA mole ratios.		*# Step two was repeated with Tris buffer at concentrations of 1 mM, 100 μM, and 10 μM on fibers with arbitrary Au/BSA mole ratios.
	*# Steps 2 and 3 were repeated with pH 10 Tris buffer.		*# Steps 2 and 3 were repeated with pH 10 Tris buffer.
		+
		+	* UV-vis spectroscopy was conducted on the resuspended fiber solutions.
		+
		+	==UV-Vis Analysis==
		+	* Graph
		+
		+	==Notes==
		+	* Refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/05\|Keyun Wang's entry]] for observations on making and centrifuging Au/BSA solutions.

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Melissa Novy: /* Au/BSA Fibers */

2012-09-05T17:49:51Z

Au/BSA Fibers

←Older revision		Revision as of 17:49, 5 September 2012
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	==Au/BSA Fibers==		==Au/BSA Fibers==
-	* Au/BSA solutions were centrifuged at 3000 rpm, 25°C, for 5 min	+	* Protocol for Resuspending Au/BSA Fibers
		+	*# Au/BSA solutions were centrifuged at 3000 rpm, 25°C, for 5 min, such that the fibers formed a pellet at the bottom of the centrifuge tube.
		+	*# 1 mL of 10 mM pH 8 Tris buffer was added to each pellet and pipetted up and down to resuspend the fibers.
		+	*# Step two was repeated with Tris buffer at concentrations of 1 mM, 100 μM, and 10 μM on fibers with arbitrary Au/BSA mole ratios.
		+	*# Steps 2 and 3 were repeated with pH 10 Tris buffer.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->