User:Melissa Novy/Notebook/CHEM-571/2012/09/18

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==Entry title==
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==0.2 M Sodium Phosphate Buffer in H<sub>2</sub>O==
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* Insert content here...
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* Calculations:
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** 250 mL of 0.2 M sodium phosphate stock solution requires the following:
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  '''MW sodium phosphate''': 163.94 g/mol
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  '''Mass sodium phosphate''':
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        250 mL × (10<sup>-3</sup> L/mL) × 0.2 M × (10<sup>-3</sup> mol/mmol) × 163.94 g/mol = 13.4035 g sodium phosphate
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  '''Actual mass sodium phosphate''': 13.4031 g
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  '''Actual concentration''':
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        13.4031 g × (1 mol/163.94 g) × (1/0.25 L) = 0.327 M in H<sub>2</sub>O
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* 13.4031 g of sodium phosphate was dissolved in about 200 mL of H<sub>2</sub>O.
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* The pH was adjusted to 7.00 by adding 20 mL of 1 N HCl dropwise.
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* The solution was then diluted to a final volume of 250 mL with additional H<sub>2</sub>O.

Revision as of 14:53, 18 September 2012

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0.2 M Sodium Phosphate Buffer in H2O

  • Calculations:
    • 250 mL of 0.2 M sodium phosphate stock solution requires the following:
  MW sodium phosphate: 163.94 g/mol
  Mass sodium phosphate:
        250 mL × (10-3 L/mL) × 0.2 M × (10-3 mol/mmol) × 163.94 g/mol = 13.4035 g sodium phosphate
  Actual mass sodium phosphate: 13.4031 g
  Actual concentration: 
        13.4031 g × (1 mol/163.94 g) × (1/0.25 L) = 0.327 M in H2O
  • 13.4031 g of sodium phosphate was dissolved in about 200 mL of H2O.
  • The pH was adjusted to 7.00 by adding 20 mL of 1 N HCl dropwise.
  • The solution was then diluted to a final volume of 250 mL with additional H2O.



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