User:Melissa Novy/Notebook/CHEM-571/2012/09/18

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* The solution was then diluted to a final volume of 250 mL with additional H<sub>2</sub>O.
* The solution was then diluted to a final volume of 250 mL with additional H<sub>2</sub>O.
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==Spectrophotometric Analysis of Horseradish Peroxidase Enzyme Activity Rate at 510 nm==
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* Final concentrations of stock solutions in total volume of 1.5 mL:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Stock Solution'''
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| align="center" style="background:#f0f0f0;"|'''Initial Concentration, M<sub>1</sub> [M]'''
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| align="center" style="background:#f0f0f0;"|'''Volume Added V<sub>1</sub> [mL]'''
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| align="center" style="background:#f0f0f0;"|'''Final Concentration, M<sub>2</sub> [M]'''
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|-
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| H<sub>2</sub>O<sub>2</sub>||0.000034||0.75||0.000017
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|-
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| AAP||0.0025||0.7||0.001166667
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|-
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| Phenol||0.018||0.01||0.00012
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|-
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|}
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Revision as of 15:19, 18 September 2012

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0.2 M Sodium Phosphate Buffer in H2O

  • Calculations:
    • 250 mL of 0.2 M sodium phosphate stock solution requires the following:
  MW sodium phosphate: 163.94 g/mol
  Mass sodium phosphate:
        250 mL × (10-3 L/mL) × 0.2 M × (10-3 mol/mmol) × 163.94 g/mol = 13.4035 g sodium phosphate
  Actual mass sodium phosphate: 13.4031 g
  Actual concentration: 
        13.4031 g × (1 mol/163.94 g) × (1/0.25 L) = 0.327 M in H2O
  • 13.4031 g of sodium phosphate was dissolved in about 200 mL of H2O.
  • The pH was adjusted to 7.00 by adding 20 mL of 1 N HCl dropwise.
  • The solution was then diluted to a final volume of 250 mL with additional H2O.

Spectrophotometric Analysis of Horseradish Peroxidase Enzyme Activity Rate at 510 nm

  • Final concentrations of stock solutions in total volume of 1.5 mL:
Stock Solution Initial Concentration, M1 [M] Volume Added V1 [mL] Final Concentration, M2 [M]
H2O20.0000340.750.000017
AAP0.00250.70.001166667
Phenol0.0180.010.00012


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