User:Melissa Novy/Notebook/CHEM-571/2012/09/18

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0.2 M Sodium Phosphate Buffer in H2O

  • Calculations:
    • 250 mL of 0.2 M sodium phosphate stock solution requires the following:
  MW sodium phosphate: 163.94 g/mol
  Mass sodium phosphate:
        250 mL × (10-3 L/mL) × 0.2 M × (10-3 mol/mmol) × 163.94 g/mol = 13.4035 g sodium phosphate
  Actual mass sodium phosphate: 13.4031 g
  Actual concentration: 
        13.4031 g × (1 mol/163.94 g) × (1/0.25 L) = 0.327 M in H2O
  • 13.4031 g of sodium phosphate was dissolved in about 200 mL of H2O.
  • The pH was adjusted to 7.00 by adding 20 mL of 1 N HCl dropwise.
  • The solution was then diluted to a final volume of 250 mL with additional H2O.



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