User:Melissa Novy/Notebook/CHEM-571/2012/09/19/2012/09/25: Difference between revisions

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==Luminol Stock Solution in Carbonate Buffer==
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==
* Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19|entry on 2012/09/19]] for luminol stock solution protocol and calculations.
* Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19|entry on 2012/09/19]] for luminol stock solution protocol and calculations.
* Actual mass luminol: 0.00443 g
* Actual mass luminol: 0.00443 g

Revision as of 10:20, 25 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Objectives

  • Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.

Luminol Stock Solution in H2O and Carbonate Buffer

  • Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
  • Actual mass luminol: 0.00443 g
  • Actual concentration luminol: 2.5 mM
  • Actual mass Na2CO3: 0.08 g
  • Actual mass naHCO3: 0.48 g

Horseradish Peroxidase Chemiluminescence Assay

  • Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.
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