User:Melissa Novy/Notebook/CHEM-571/2012/09/19/2012/09/25: Difference between revisions

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==Entry title==
==Objectives==
* Insert content here...
* Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.
* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
 
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==
* Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19|entry on 2012/09/19]] for luminol stock solution protocol and calculations.
** Actual mass luminol: 0.00443 g
** Actual concentration luminol: 2.5 mM
** Actual mass Na<sub>2</sub>CO<sub>3</sub>: 0.08 g
** Actual mass naHCO<sub>3</sub>: 0.48 g
 
==Horseradish Peroxidase Chemiluminescence Assay==
* Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.
 




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Revision as of 10:41, 25 September 2012

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