User:Melissa Novy/Notebook/CHEM-571/2012/09/25

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* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
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** Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/25|Keyun Wang's entry]] for actual masses and volumes.
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==

Revision as of 12:46, 25 September 2012

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Objectives

Luminol Stock Solution in H2O and Carbonate Buffer

  • Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
    • Actual mass luminol: 0.00443 g
    • Actual concentration luminol: 2.5 mM
    • Actual mass Na2CO3: 0.08 g
    • Actual mass naHCO3: 0.48 g

Horseradish Peroxidase Chemiluminescence Assay

  • Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.




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