User:Melissa Novy/Notebook/CHEM-571/2012/09/25

Objectives

• Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.
• Make starter culture media and expression culture media to use with E. coli cells expressing adenosine deaminase enzyme.
  • No alterations were made to the starter culture media protocol or the expression culture media protocol.
  • The media were then autoclaved according to the procedure.
  • Please refer to Keyun Wang's entry for actual masses and volumes used to make the media.

Luminol Stock Solution in H₂O and Carbonate Buffer

• Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
  • Actual mass luminol: 0.00443 g
  • Actual concentration luminol: 2.5 mM
  • Actual mass Na₂CO₃: 0.08 g
  • Actual mass naHCO₃: 0.48 g

Horseradish Peroxidase Chemiluminescence Assay

• Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.