User:Melissa Novy/Notebook/CHEM-571/2012/09/26

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(Spectroscopy Data of Tris Buffers)
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==Spectroscopy Data of Tris Buffers==
==Spectroscopy Data of Tris Buffers==
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Graph goes here.
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[[Image:Tris_Buffer_Absorbance.JPG]]
* Graph of absorbance versus wavelength of Tris buffer in H<sub>2</sub>O at pH 8 and 10 and at concentrations ranging from 10 μM to 10 mM.
* Graph of absorbance versus wavelength of Tris buffer in H<sub>2</sub>O at pH 8 and 10 and at concentrations ranging from 10 μM to 10 mM.
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* Note that very small peaks were observed around 730-740 nm for all solutions and another small, broad peak was observed around 600 nm for the 10 μM Tris buffer solution at pH 8.  The data indicate that absorbance of Tris buffer does not affect the peak observed for AuNPs at around 540 nm and the concentration and pH of Tris does not significantly affect the peaks for Tris observed.
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Revision as of 14:48, 26 September 2012

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Objectives

  • Continue optimizing the HRP chemiluminescence assay by adjusting the concentrations of the reactants.
  • Analyze Tris buffers at pH 8 and 10 and at various concentrations with UV-vis spectroscopy.

Spectroscopy Data of Tris Buffers

Image:Tris_Buffer_Absorbance.JPG

  • Graph of absorbance versus wavelength of Tris buffer in H2O at pH 8 and 10 and at concentrations ranging from 10 μM to 10 mM.
  • Note that very small peaks were observed around 730-740 nm for all solutions and another small, broad peak was observed around 600 nm for the 10 μM Tris buffer solution at pH 8. The data indicate that absorbance of Tris buffer does not affect the peak observed for AuNPs at around 540 nm and the concentration and pH of Tris does not significantly affect the peaks for Tris observed.


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