User:Melissa Novy/Notebook/CHEM-571/2012/10/02 - Revision history

Melissa Novy at 20:14, 26 November 2012

Melissa Novy — Mon, 26 Nov 2012 20:14:22 GMT

←Older revision		Revision as of 20:14, 26 November 2012
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	==Objectives==		==Objectives==
	* Obtain proteins from cells prepared on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/26\|2012/09/26]].		* Obtain proteins from cells prepared on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/26\|2012/09/26]].
-	** Adenosine deaminase (ADA) will be separated from the rest of the cell proteins on 2012/10/03.	+	** Adenosine deaminase (ADA) will be separated from the rest of the cell proteins on [[User:Melissa_Novy/Notebook/CHEM-571/2012/10/03\|2012/10/03]].

	==Protein Purification==		==Protein Purification==

Melissa Novy at 03:20, 3 October 2012

Melissa Novy — Wed, 03 Oct 2012 03:20:59 GMT

←Older revision		Revision as of 03:20, 3 October 2012
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-	==~~Entry title~~==	+	==Objectives==
-	* ~~Insert content here.~~..	+	* Obtain proteins from cells prepared on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/26\|2012/09/26]].
		+	** Adenosine deaminase (ADA) will be separated from the rest of the cell proteins on 2012/10/03.

		+	==Protein Purification==
		+	* The cells were lysed using a Misonix XL-2000 sonicator to allow direct manipulation of organelles, proteins, and other cell components in solution.
		+	* The sonicated cell solution was centrifuged at 4°C and 18,000 rpm for a period of 2 hours.
		+	** Note that cooling the solutions prevents cell proteins from denaturing.
		+	** The resultant supernatant was then filtered with a 450 nm nylon filter to remove all large cell components. The filtrate is expected to contain cell proteins.
		+	* The filtrate containing cell proteins, including ADA, was refrigerated at 4°C until needed.
		+	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/02\|Dhea Patel's entry]] for the detailed protocol for protein purification and proper disposal of biowaste.

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Melissa Novy: Autocreate 2012/10/02 Entry for User:Melissa_Novy/Notebook/CHEM-571

Melissa Novy — Wed, 03 Oct 2012 02:39:24 GMT

Autocreate 2012/10/02 Entry for User:Melissa_Novy/Notebook/CHEM-571

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