User:Melissa Novy/Notebook/CHEM-571/2012/10/03

Objectives

• Use fast protein liquid chromatography (FPLC) on the cell protein solution made on 2012/10/02 to obtain ADA.
• Make 10 mL of Au/BSA solutions at the usual mole ratios (60 through 170 Au:BSA) for atomic absorption. Please refer to the following table for more information.

• Abigail E. Miller 18:19, 7 October 2012 (EDT):where is the information about separating the ADA? how was it done? what conditions and parameter were important? what buffers were used?

Au/BSA Solutions for AA

• Due to a miscalculation, the stock solutions for HAuCl₄ and for BSA were combined. The total number of moles of BSA and moles of HAuCl₄ in this new solution were calculated and then new volumes of BSA stock solution were determined:

• Abigail E. Miller 18:18, 7 October 2012 (EDT):give what you initially created to then combine. what was the original stock solution. what volumes of gold nad BSA were mixed before combining?

• There are 0.00000208 mol of HAuCl₄ per 0.00008 L of solution.
• There are 2.4E-10 mol of BSA per 0.00008 L of solution.
• Please refer to the following table for calculations and concentrations:

• The protocol for making the AA Au/BSA solutions is as follows:
  1. Add 80 μL of Au/BSA stock solution to each test tube.
  2. Then add the given volumes of BSA stock solution to each test tube (refer to Volume BSA Added [μL]).
  3. Dilute all solutions to a final volume of 10 mL.
  4. Place the solutions in an 80°C oven for 4 hours.