User:Melissa Novy/Notebook/CHEM-571/2012/10/09 - Revision history

Melissa Novy: /* AAS Protocol */

2012-12-07T04:22:55Z

AAS Protocol

←Older revision		Revision as of 04:22, 7 December 2012
Line 20:		Line 20:

	==Atomic Absorption of Au/BSA Solutions==		==Atomic Absorption of Au/BSA Solutions==
-	~~===AAS Protocol===~~
	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.		* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
	* Deionized water was run through the AA between samples.		* Deionized water was run through the AA between samples.
-	~~<br><br>~~
	* Au/BSA solutions containing fibers at Au:BSA mole ratios of 120 through 170 were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.		* Au/BSA solutions containing fibers at Au:BSA mole ratios of 120 through 170 were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-12-07T04:22:28Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 04:22, 7 December 2012
Line 20:		Line 20:

	==Atomic Absorption of Au/BSA Solutions==		==Atomic Absorption of Au/BSA Solutions==
		+	===AAS Protocol===
	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.		* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
	* Deionized water was run through the AA between samples.		* Deionized water was run through the AA between samples.
		+	<br><br>
	* Au/BSA solutions containing fibers at Au:BSA mole ratios of 120 through 170 were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.		* Au/BSA solutions containing fibers at Au:BSA mole ratios of 120 through 170 were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-12-02T19:32:46Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 19:32, 2 December 2012
Line 22:		Line 22:
	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.		* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
	* Deionized water was run through the AA between samples.		* Deionized water was run through the AA between samples.
-	* Au/BSA solutions containing fibers at Au:BSA mole ratios of ~~(note ratios here)~~ were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.	+	* Au/BSA solutions containing fibers at Au:BSA mole ratios of 120 through 170 were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.
	** Colored solutions at Au:BSA mole ratios of 120, 128, and 130 that contained fibers produced clear filtrate after being filtered and the AA detected that they contained little to no gold (please refer to Table 1). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.		** Colored solutions at Au:BSA mole ratios of 120, 128, and 130 that contained fibers produced clear filtrate after being filtered and the AA detected that they contained little to no gold (please refer to Table 1). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-11-26T21:16:10Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 21:16, 26 November 2012
Line 62:		Line 62:
	\| 170\|\|40.9690424\|\|-0.2845\|\|41.2535424\|\|100.6944268		\| 170\|\|40.9690424\|\|-0.2845\|\|41.2535424\|\|100.6944268
	\|}		\|}
		+	<br>
		+	[[Image:Measured_Au_ppm.jpg]]
		+	<br><br>
		+	[[Image:Change_in_Au_ppm.jpg]]
		+	<br><br>
		+	* Note that as negative concentrations of Au are experimentally impossible, the values are likely due to improper zeroing of the AAS. For the purposes of data analysis, it will be assumed that the concentration of Au in those solutions is zero.
		+	* According to Table 1, Au/BSA solutions at mole ratios of 60, 80, 100, 132, 133, and 134 contained Au. These solutions likely successfully formed AuNPs, corroborated by visual observation of their purple color. The remainder of the solutions likely did not form AuNPs, as no Au was detected by the AAS. Thus, it can be assumed that BSA encapsulated the Au added to the solutions and thereby prevented Au from being present in solution. However, it is also possible that filtration removed AuNPs from solution, as in the case of solutions of Au:BSA mole ratios of 136 through 170.
	<br>		<br>
	* Table 2: Change in Moles of Au of Solutions of Au/BSA		* Table 2: Change in Moles of Au of Solutions of Au/BSA
Line 100:		Line 107:
	\| 170\|\|2.08E-06\|\|-1.44441E-06\|\|-1.44441E-08\|\|2.09E-06\|\|100.6944268		\| 170\|\|2.08E-06\|\|-1.44441E-06\|\|-1.44441E-08\|\|2.09E-06\|\|100.6944268
	\|}		\|}
		+	<br>
		+	[[Image:Change_in_Mol_Au.jpg]]

	==Activity of HRP Based on the HRP-AAP Assay==		==Activity of HRP Based on the HRP-AAP Assay==

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-11-26T20:49:24Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 20:49, 26 November 2012
Line 22:		Line 22:
	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.		* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
	* Deionized water was run through the AA between samples.		* Deionized water was run through the AA between samples.
-	* Au/BSA solutions containing fibers (note ~~which~~ ratios here) were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.	+	* Au/BSA solutions containing fibers at Au:BSA mole ratios of (note ratios here) were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.
-	** Colored solutions ~~(note~~ ratios ~~here) containing fiber~~ produced clear filtrate after being filtered and the AA detected that they contained little to no gold (~~note ppm values here~~). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.	+	** Colored solutions at Au:BSA mole ratios of 120, 128, and 130 that contained fibers produced clear filtrate after being filtered and the AA detected that they contained little to no gold (please refer to Table 1). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.
		+	<br>
		+	* Table 1: Change in Au Concentration (ppm) of Solutions of Au/BSA
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Au:BSA Mole Ratio'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Theoretical Conc. Au [ppm]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Actual Conc. Au [ppm]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Δ Au Conc. [ppm]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Percent Change Conc. Au [%]'''
		+	\|-
		+	\| 60\|\|40.9690424\|\|50.1489\|\|-9.1798576\|\|-22.40681515
		+	\|-
		+	\| 80\|\|40.9690424\|\|50.5319\|\|-9.5628576\|\|-23.34166737
		+	\|-
		+	\| 100\|\|40.9690424\|\|52.6358\|\|-11.6667576\|\|-28.47700829
		+	\|-
		+	\| 120\|\|40.9690424\|\|-0.77\|\|41.7390424\|\|101.8794679
		+	\|-
		+	\| 128\|\|40.9690424\|\|-0.3222\|\|41.2912424\|\|100.7864475
		+	\|-
		+	\| 130\|\|40.9690424\|\|-0.3654\|\|41.3344424\|\|100.8918929
		+	\|-
		+	\| 132\|\|40.9690424\|\|35.2115\|\|5.7575424\|\|14.05339755
		+	\|-
		+	\| 133\|\|40.9690424\|\|35.0928\|\|5.8762424\|\|14.34312851
		+	\|-
		+	\| 134\|\|40.9690424\|\|33.8251\|\|7.1439424\|\|17.43741611
		+	\|-
		+	\| 136\|\|40.9690424\|\|-0.8617\|\|41.8307424\|\|102.1032954
		+	\|-
		+	\| 138\|\|40.9690424\|\|-0.7862\|\|41.7552424\|\|101.9190099
		+	\|-
		+	\| 140\|\|40.9690424\|\|-0.8185\|\|41.7875424\|\|101.99785
		+	\|-
		+	\| 160\|\|40.9690424\|\|-0.6891\|\|41.6581424\|\|101.6820017
		+	\|-
		+	\| 170\|\|40.9690424\|\|-0.2845\|\|41.2535424\|\|100.6944268
		+	\|}
		+	<br>
		+	* Table 2: Change in Moles of Au of Solutions of Au/BSA
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Au:BSA Mole Ratio'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Theoretical Mol Au [mol]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Actual Conc. Au [M]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Actual Mol Au [mol]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Δ Mol Au [mol]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Percent Change Mol Au [%]'''
		+	\|-
		+	\| 60\|\|2.08E-06\|\|0.000254606\|\|2.54606E-06\|\|-4.66E-07\|\|-22.40681515
		+	\|-
		+	\| 80\|\|2.08E-06\|\|0.000256551\|\|2.56551E-06\|\|-4.86E-07\|\|-23.34166737
		+	\|-
		+	\| 100\|\|2.08E-06\|\|0.000267232\|\|2.67232E-06\|\|-5.92E-07\|\|-28.47700829
		+	\|-
		+	\| 120\|\|2.08E-06\|\|-3.90929E-06\|\|-3.90929E-08\|\|2.12E-06\|\|101.8794679
		+	\|-
		+	\| 128\|\|2.08E-06\|\|-1.63581E-06\|\|-1.63581E-08\|\|2.10E-06\|\|100.7864475
		+	\|-
		+	\| 130\|\|2.08E-06\|\|-1.85514E-06\|\|-1.85514E-08\|\|2.10E-06\|\|100.8918929
		+	\|-
		+	\| 132\|\|2.08E-06\|\|0.000178769\|\|1.78769E-06\|\|2.92E-07\|\|14.05339755
		+	\|-
		+	\| 133\|\|2.08E-06\|\|0.000178166\|\|1.78166E-06\|\|2.98E-07\|\|14.34312851
		+	\|-
		+	\| 134\|\|2.08E-06\|\|0.00017173\|\|1.7173E-06\|\|3.63E-07\|\|17.43741611
		+	\|-
		+	\| 136\|\|2.08E-06\|\|-4.37485E-06\|\|-4.37485E-08\|\|2.12E-06\|\|102.1032954
		+	\|-
		+	\| 138\|\|2.08E-06\|\|-3.99154E-06\|\|-3.99154E-08\|\|2.12E-06\|\|101.9190099
		+	\|-
		+	\| 140\|\|2.08E-06\|\|-4.15553E-06\|\|-4.15553E-08\|\|2.12E-06\|\|101.99785
		+	\|-
		+	\| 160\|\|2.08E-06\|\|-3.49856E-06\|\|-3.49856E-08\|\|2.11E-06\|\|101.6820017
		+	\|-
		+	\| 170\|\|2.08E-06\|\|-1.44441E-06\|\|-1.44441E-08\|\|2.09E-06\|\|100.6944268
		+	\|}

	==Activity of HRP Based on the HRP-AAP Assay==		==Activity of HRP Based on the HRP-AAP Assay==

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-10-29T01:27:50Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 01:27, 29 October 2012
Line 22:		Line 22:
	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.		* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
	* Deionized water was run through the AA between samples.		* Deionized water was run through the AA between samples.
-	* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.	+	* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry 0.45 μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.
	** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.		** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.

Melissa Novy: /* Activity of HRP Based on the HRP-AAP Assay= */

2012-10-29T01:25:47Z

Activity of HRP Based on the HRP-AAP Assay=

←Older revision		Revision as of 01:25, 29 October 2012
Line 26:		Line 26:
	** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.		** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.

-	==Activity of HRP Based on the HRP-AAP Assay===	+	==Activity of HRP Based on the HRP-AAP Assay==
	* Note that data obtained on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/18\|2012/09/18]] was used to calculate the activity of HRP.		* Note that data obtained on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/18\|2012/09/18]] was used to calculate the activity of HRP.
	* The ratio of the change in time to the change in absorbance was calculated to be the rate of the reaction. The inverse of the rate was plotted against the inverse of the concentration of whichever reactant was varied, either AAP or H<sub>2</sub>O<sub>2</sub>.		* The ratio of the change in time to the change in absorbance was calculated to be the rate of the reaction. The inverse of the rate was plotted against the inverse of the concentration of whichever reactant was varied, either AAP or H<sub>2</sub>O<sub>2</sub>.

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-10-26T05:44:07Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 05:44, 26 October 2012
Line 24:		Line 24:
	* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.		* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.
-	** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is	+	** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is due to the optical properties of gold nanoparticles.
-	due to the optical properties of gold nanoparticles.	+
		+	==Activity of HRP Based on the HRP-AAP Assay===
		+	* Note that data obtained on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/18\|2012/09/18]] was used to calculate the activity of HRP.
		+	* The ratio of the change in time to the change in absorbance was calculated to be the rate of the reaction. The inverse of the rate was plotted against the inverse of the concentration of whichever reactant was varied, either AAP or H<sub>2</sub>O<sub>2</sub>.
		+	<br>
		+	[[Image:Varying_AAP.jpg]]
		+	* Note that the concentration of H<sub>2</sub>O<sub>2</sub> was fixed at 1.7 mM.
		+	<br>
		+	[[Image:Varying_H2O2.jpg]]
		+	* Note that the concentration of AAP was fixed at 2.5 mM.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Melissa Novy: /* Atomic Absorption of Au/BSA Solutions */

2012-10-10T12:05:52Z

Atomic Absorption of Au/BSA Solutions

←Older revision		Revision as of 12:05, 10 October 2012
Line 20:		Line 20:

	==Atomic Absorption of Au/BSA Solutions==		==Atomic Absorption of Au/BSA Solutions==
-	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]].	+	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]] and blanked with 1 N HCl.
		+	* Deionized water was run through the AA between samples.
	* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.		* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.		** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.

Melissa Novy at 12:05, 10 October 2012

2012-10-10T12:05:09Z

←Older revision		Revision as of 12:05, 10 October 2012
Line 15:		Line 15:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	==~~Entry title~~==	+	==Objectives==
-	* ~~Insert content here.~~..	+	* Analyze Au/BSA solutions made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/10/03\|2012/10/03]] with atomic absorption.
		+	* Calculate activity of HRP from the UV-vis data obtained from the HRP-AAP assay on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/18\|2012/09/18]] and [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19\|2012/09/19]].

		+	==Atomic Absorption of Au/BSA Solutions==
		+	* The AA was calibrated with standard solutions at concentrations of 5, 8, 10, 15, 20, 25, 30, and 40 ppm made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/10/08\|2012/10/08]].
		+	* Au/BSA solutions containing fibers (note which ratios here) were filtered with dry x μM pore-size nylon filter paper into a clean, dry flask. The filtrate was then decanted into a Falcon tube for testing.
		+	** Note that the fibers on the filter paper were not washed. The fibers on the filter paper were dark purple in color.
		+	** Colored solutions (note ratios here) containing fiber produced clear filtrate after being filtered and the AA detected that they contained little to no gold (note ppm values here). This indicates that either the gold was of large enough size to be filtered by the filter paper, or that the pores in the filter paper were blocked with fibers and thus did not filter the gold. It would be incorrect to assume that the solutions did not contain any free gold, as literature states that the purple color of the solutions before filtering is
		+	due to the optical properties of gold nanoparticles.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
-	\|}	+

	__NOTOC__		__NOTOC__