User:Melissa Novy/Notebook/CHEM-572/2013/02/06

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==Objectives==
==Objectives==
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* Make 50 mL 5 M KNO<sub>3</sub> solution for ionic strength adjustments with silver ion-selective electrode.
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* Make 50 mL 5 M [http://www.sciencelab.com/msds.php?msdsId=9927232| KNO<sub>3</sub>] solution for ionic strength adjustments with silver ion-selective electrode.
-
* Make LB/agar solution.
+
* Make agar solution and plate ''E. coli'' cells.
 +
* Analyze cells with UV-vis spectrophotometry.
* Make PLA2002D + 5wt% Laponite (PLA + 5LMT) using dichloromethane.
* Make PLA2002D + 5wt% Laponite (PLA + 5LMT) using dichloromethane.
 +
* Analyze XRD data obtained on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|2013/02/05]].
 +
 +
==XRD on 95AgLMT==
 +
* Laponite
 +
[[Image:LMT_2-6-13.JPG]]
 +
<br><br>
 +
 +
* 95AgLMT stirred for 24 h and made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/30|2013/01/30]].
 +
[[Image:AgLMT_24h_2-6-13.JPG]]
 +
<br><br>
 +
 +
* List of peaks.  Analysis.
 +
 +
==Agar Solution and Agar Plates==
 +
* The plates made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/30|2013/01/30]] were unusuable, so the plates were remade using the same protocol as before.
 +
* However, modifications to the recipe were as follows:
 +
** 12.5 g Teknova LB medium
 +
** 7.5 g ??? agar
==Calculations for 5 M KNO<sub>3</sub>==
==Calculations for 5 M KNO<sub>3</sub>==
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* Actual mass KNO<sub>3</sub>: 25.2756 g
* Actual mass KNO<sub>3</sub>: 25.2756 g
 +
* Actual molarity KNO<sub>3</sub>: 5.00 M
* KNO<sub>3</sub> was placed in a glass screw-top bottle and 50 mL of pure H<sub>2</sub>O was added.  The solution was stirred and heated at 70°C for 3 hours to dissolve the KNO<sub>3</sub>.
* KNO<sub>3</sub> was placed in a glass screw-top bottle and 50 mL of pure H<sub>2</sub>O was added.  The solution was stirred and heated at 70°C for 3 hours to dissolve the KNO<sub>3</sub>.
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* The solution was stirred for 1 h and then cast into a Teflon dish.
* The solution was stirred for 1 h and then cast into a Teflon dish.
* The dish was left under the fume hood to encourage evaporation of the dichloromethane.
* The dish was left under the fume hood to encourage evaporation of the dichloromethane.
 +
 +
==UV-Vis on ''E. coli'' Cells==
 +
* Absorbance was measured at 650 nm with a Shimadzu UV-1800 spectrophotometer using plastic cuvettes.  2 mL of each sample was pipetted into a clean plastic cuvette.
 +
* The absorbance of DH5α was 1.800.
 +
* The absorbance of DH10B was 1.82.

Revision as of 15:12, 12 February 2013

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Objectives

  • Make 50 mL 5 M KNO3 solution for ionic strength adjustments with silver ion-selective electrode.
  • Make agar solution and plate E. coli cells.
  • Analyze cells with UV-vis spectrophotometry.
  • Make PLA2002D + 5wt% Laponite (PLA + 5LMT) using dichloromethane.
  • Analyze XRD data obtained on 2013/02/05.

XRD on 95AgLMT

  • Laponite

Image:LMT_2-6-13.JPG

  • 95AgLMT stirred for 24 h and made on 2013/01/30.

Image:AgLMT_24h_2-6-13.JPG

  • List of peaks. Analysis.

Agar Solution and Agar Plates

  • The plates made on 2013/01/30 were unusuable, so the plates were remade using the same protocol as before.
  • However, modifications to the recipe were as follows:
    • 12.5 g Teknova LB medium
    • 7.5 g ??? agar

Calculations for 5 M KNO3

  MW KNO3: 101.10 g/mol
  0.050 L × 5 M KNO3 = 0.25 mol KNO3
  0.25 mol KNO3 × (101.10 g/1 mol KNO3) = 25.275 g KNO3
  • Actual mass KNO3: 25.2756 g
  • Actual molarity KNO3: 5.00 M
  • KNO3 was placed in a glass screw-top bottle and 50 mL of pure H2O was added. The solution was stirred and heated at 70°C for 3 hours to dissolve the KNO3.

PLA2002D + 5wt% Laponite Film

  • 1 g of PLA was dissolved in 10 mL of dichloromethane in a fume hood.
  • 0.05 g Laponite was dispersed in 5 mL of dichloromethane, and then added to the PLA solution.
  • The solution was stirred for 1 h and then cast into a Teflon dish.
  • The dish was left under the fume hood to encourage evaporation of the dichloromethane.

UV-Vis on E. coli Cells

  • Absorbance was measured at 650 nm with a Shimadzu UV-1800 spectrophotometer using plastic cuvettes. 2 mL of each sample was pipetted into a clean plastic cuvette.
  • The absorbance of DH5α was 1.800.
  • The absorbance of DH10B was 1.82.



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