User:Melissa Novy/Notebook/CHEM-572/2013/02/13

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** Make 0.10 M AgNO<sub>3</sub> solution.
** Make 0.10 M AgNO<sub>3</sub> solution.
* Remove PLA2002D + 5 wt % LMT film from glass mold.
* Remove PLA2002D + 5 wt % LMT film from glass mold.
 +
* Prepare a starter culture of DH5α-T1 cells.
 +
** Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/13|Keyun Wang's entry]] for the protocol.
==0.1 M AgNO<sub>3</sub> Solution==
==0.1 M AgNO<sub>3</sub> Solution==
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|}
|}
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[[Image:AgCC.png]]
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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* The error bars indicate a 5% deviation.
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<br>
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* Conductivity of filtrate solutions
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample []'''
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| align="center" style="background:#f0f0f0;"|'''Conductivity [mV]'''
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| align="center" style="background:#f0f0f0;"|'''Average [mV]'''
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| align="center" style="background:#f0f0f0;"|'''log [Ag+] []'''
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| align="center" style="background:#f0f0f0;"|'''[Ag+] [M]'''
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|-
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| (50:50) H2O/EtOH||372||374.3333333||-3.894305004||0.000127554
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|-
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| ||377.8||||||
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|-
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| ||373.2||||||
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|-
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| [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/12|100Ag-LMT Filtrate]]||403.6||389.7333333||-3.595826469||0.000253614
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|-
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| ||389.3||||||
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|-
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| ||376.3||||||
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|-
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| [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/30|95Ag-LMT Filtrate]]||582.3||582.7666667||0.145492134||1.397951596
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|-
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| ||582.9||||||
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|-
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| ||583.1||||||
|}
|}
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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__NOTOC__
__NOTOC__

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Objectives

  • Make a calibration curve for the determination of Ag+ in solutions.
    • Make 0.10 M AgNO3 solution.
  • Remove PLA2002D + 5 wt % LMT film from glass mold.
  • Prepare a starter culture of DH5α-T1 cells.

0.1 M AgNO3 Solution

  • Calculations 
  250 mL of 0.1 M AgNO3
  MW AgNO3 = 169.87 g/mol
  0.1 M × 0.250 L = 0.025 mol
  0.025 mol AgNO3 × (169.87 g AgNO3/1 mol AgNO3) = 4.24675 g AgNO3
  • Actual mass AgNO3: 4.24694 g
  • Actual molarity AgNO3: 0.10000 M AgNO3

Ag+ Ion Selective Electrode Calibration

  • The protocol from 2013/02/12 was followed using solutions of the following [AgNO3]:
    • 0 M AgNO3
    • 0.0001 M AgNO3
    • 0.0010 M AgNO3
    • 0.0105 M AgNO3
    • 0.1000M AgNO3
  • Conductivity of solutions of AgNO3
[Ag+] [M] log [Ag+] [] Conductivity [mV]
0.000000394.6
0.000000390.7
0.000000386.4
0.00010-4377
0.00010-4375.2
0.00010-4374.7
0.00100-3411.6
0.00100-3411.8
0.00100-3411.8
0.01050-1.98470.1
0.01050-1.98470.3
0.01050-1.98470.3
0.10000-1528.5
0.10000-1528.5
0.10000-1528.5

Image:AgCC.png

  • The error bars indicate a 5% deviation.


  • Conductivity of filtrate solutions
Sample [] Conductivity [mV] Average [mV] log [Ag+] [] [Ag+] [M]
(50:50) H2O/EtOH372374.3333333-3.8943050040.000127554
377.8
373.2
100Ag-LMT Filtrate403.6389.7333333-3.5958264690.000253614
389.3
376.3
95Ag-LMT Filtrate582.3582.76666670.1454921341.397951596
582.9
583.1



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