User:Melissa Novy/Notebook/CHEM-572/2013/02/20

Objectives

• Calculate the amount of AgNO₃ to add to LB media solutions for desired final [Ag⁺].
• Incubate the silver-doped LB media solutions for 15 hours to monitor bacterial growth.
• Remove PLA films, made on 2013/02/19, from glass molds.
• Soak PLA films in pure H₂O to test for Ag⁺ leaching with a silver ion selective electrode.

Calculations

• Two sample calculations are given as examples. The final [Ag⁺] in 50-mL solutions of LB media was calculated as follows:

  1 μM Ag+ in 50 mL of LB media
  (0.0105 M Ag+) × V1 = (1×10-6 M) × (50 mL)
  V1 = 0.00476 mL of 0.0105 M AgNO3

  10 μM Ag+ in 50 mL of LB media
  (0.1 M Ag+) × V1 = (10×10-6 M) × (50 mL)
  V1 = 0.005 mL of 0.1 M AgNO3

• AgNO₃ solutions made on 2013/02/05 and 2013/02/13 were added to the LB media solutions in the following amounts:

• Note that for each volume of AgNO₃ added to the LB media, the same volume of LB media was first removed from the flask to maintain the final concentration of Ag⁺.